Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   H9K75_RS11230 Genome accession   NZ_CP060783
Coordinates   2407355..2408950 (-) Length   531 a.a.
NCBI ID   WP_187725801.1    Uniprot ID   A0A7H0GPS8
Organism   Diaphorobacter aerolatus strain KACC 16536     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2402355..2413950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9K75_RS11220 (H9K75_11220) - 2402441..2403367 (+) 927 WP_187725799.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  H9K75_RS11225 (H9K75_11225) - 2403379..2405829 (-) 2451 WP_187725800.1 IPTL-CTERM sorting domain-containing protein -
  H9K75_RS11230 (H9K75_11230) rpoN/rpoN1 2407355..2408950 (-) 1596 WP_187725801.1 RNA polymerase factor sigma-54 Machinery gene
  H9K75_RS11235 (H9K75_11235) lptB 2409060..2409836 (-) 777 WP_187726003.1 LPS export ABC transporter ATP-binding protein -
  H9K75_RS11240 (H9K75_11240) lptA 2409901..2410503 (-) 603 WP_226858655.1 lipopolysaccharide transport periplasmic protein LptA -
  H9K75_RS11245 (H9K75_11245) - 2410717..2411370 (-) 654 WP_187725803.1 thiol:disulfide interchange protein DsbA/DsbL -
  H9K75_RS11250 (H9K75_11250) - 2411439..2412155 (-) 717 WP_187725804.1 SPOR domain-containing protein -
  H9K75_RS11255 (H9K75_11255) argS 2412180..2413889 (-) 1710 WP_187725805.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 531 a.a.        Molecular weight: 57739.78 Da        Isoelectric Point: 4.5472

>NTDB_id=478317 H9K75_RS11230 WP_187725801.1 2407355..2408950(-) (rpoN/rpoN1) [Diaphorobacter aerolatus strain KACC 16536]
MKPGLSLRVSQHLALTPQLQQSIKLLQLSTLELEQEVEQMLDDNPFLERTTEGAEREEFGIERADQPVSEDAADADSALY
PAASASSTATSSEDQAAPDADAASAASESSEQVDWEGDGTVEMAPDDGEWGGDAPARSSTSGSGDEETDAIDLAGAHESL
SDHLHRQALALHLSDIDSAAVRFLIESLNDDGYLEESLAELAIALAGNDAPTEELEELVHRFTVGLRLLQSLDPAGVGAR
NLSECLTLQLHAMNLDEEGDPAMIAVALRICRDSLDLLARKDVKRLMQACGAGEDSTRAAMNLIARLEPRPGRRFADVQR
QIIVPDVIVKNVGRGAQMRFTVQLNPDVMPRLRVHDVYANALRGQRGNEANAGLQQRLQEARWFIKNVQQRFDTILRVST
AIVERQKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYLSTPQGTFELKYFFGSGLGTETGGSASSTAVKALIK
QLLSSESPKKPLSDSKIADMLAEQGIECARRTVAKYREALKIPPANLRKAV

Nucleotide


Download         Length: 1596 bp        

>NTDB_id=478317 H9K75_RS11230 WP_187725801.1 2407355..2408950(-) (rpoN/rpoN1) [Diaphorobacter aerolatus strain KACC 16536]
ATGAAACCCGGACTGTCGCTGCGCGTTTCGCAGCATCTGGCACTCACGCCGCAGTTGCAGCAATCGATCAAGCTGCTGCA
ACTGTCCACGCTCGAACTCGAACAGGAAGTCGAGCAAATGCTCGACGACAACCCGTTCCTGGAGCGCACGACCGAAGGCG
CGGAGCGCGAAGAATTCGGCATCGAGCGTGCGGATCAGCCCGTAAGCGAAGACGCCGCAGACGCGGACAGCGCGCTGTAT
CCGGCGGCCTCCGCGTCCAGCACCGCTACCTCGTCCGAAGATCAGGCAGCGCCGGATGCAGATGCTGCCAGCGCCGCTTC
GGAATCGTCCGAGCAGGTCGATTGGGAGGGCGACGGGACCGTCGAGATGGCCCCCGACGATGGCGAGTGGGGCGGTGACG
CTCCCGCGCGTAGCAGCACCAGCGGTTCGGGCGACGAAGAGACCGATGCCATCGATCTGGCAGGCGCGCACGAATCCCTG
AGCGACCACCTGCACCGCCAGGCGCTGGCGCTGCATTTGTCGGACATCGACAGCGCGGCGGTGCGTTTCCTGATCGAGTC
GTTGAACGACGACGGCTATCTCGAAGAGTCGCTCGCCGAACTGGCCATTGCGCTGGCCGGCAACGACGCGCCGACCGAAG
AGCTGGAAGAACTCGTGCATCGCTTCACGGTCGGCCTGCGTCTTTTGCAGAGTCTCGATCCGGCGGGTGTCGGCGCGCGC
AATCTGAGCGAGTGCCTGACGCTGCAATTGCACGCCATGAACCTCGACGAAGAGGGCGATCCGGCCATGATCGCGGTCGC
GTTGCGCATTTGCCGCGACTCGCTCGACCTGCTCGCGCGCAAGGACGTCAAGCGTCTCATGCAGGCCTGCGGCGCGGGGG
AGGATTCCACACGCGCCGCGATGAACCTGATTGCGCGCCTGGAGCCGCGTCCCGGTCGCCGGTTTGCCGACGTGCAGCGC
CAGATCATCGTGCCCGACGTGATCGTCAAGAACGTCGGTCGCGGCGCGCAGATGCGTTTTACCGTGCAACTCAATCCCGA
CGTGATGCCGCGACTGCGCGTGCACGACGTTTATGCCAACGCGCTGCGTGGCCAGCGCGGCAACGAGGCCAACGCGGGGC
TGCAGCAGCGTCTTCAGGAAGCGCGCTGGTTCATCAAGAACGTGCAGCAGCGCTTTGACACCATATTGCGCGTCTCGACC
GCCATCGTCGAGCGCCAGAAGAACTTCTTCGTGCACGGCGAACTCGCCATGCGCCCGCTGGTGCTGCGCGACATCGCCGA
TGAACTCGGCCTGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAATACCTGTCGACGCCGCAGGGCACGTTCGAGC
TCAAGTATTTCTTCGGCTCGGGCCTTGGCACCGAGACCGGCGGCAGCGCGTCGAGCACGGCGGTGAAGGCGTTGATCAAG
CAGTTGCTGAGTTCCGAAAGCCCGAAGAAGCCGCTCTCGGACAGCAAGATCGCCGACATGCTCGCCGAGCAGGGCATCGA
GTGCGCGCGTCGCACCGTGGCCAAGTACCGCGAGGCGCTGAAGATTCCTCCGGCCAATCTGCGCAAGGCGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(374-529)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(154-358)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H0GPS8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.256

100

0.524