Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   H9K76_RS01555 Genome accession   NZ_CP060714
Coordinates   323574..325154 (+) Length   526 a.a.
NCBI ID   WP_187597859.1    Uniprot ID   A0A7G9RPT6
Organism   Diaphorobacter ruginosibacter strain DSM 27467     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 318574..330154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9K76_RS01530 (H9K76_01530) argS 318712..320421 (+) 1710 WP_187597854.1 arginine--tRNA ligase -
  H9K76_RS01535 (H9K76_01535) - 320445..321152 (+) 708 WP_187597855.1 SPOR domain-containing protein -
  H9K76_RS01540 (H9K76_01540) - 321221..321871 (+) 651 WP_187597856.1 thiol:disulfide interchange protein DsbA/DsbL -
  H9K76_RS01545 (H9K76_01545) lptA 322047..322712 (+) 666 WP_187597857.1 lipopolysaccharide transport periplasmic protein LptA -
  H9K76_RS01550 (H9K76_01550) lptB 322757..323518 (+) 762 WP_187597858.1 LPS export ABC transporter ATP-binding protein -
  H9K76_RS01555 (H9K76_01555) rpoN/rpoN1 323574..325154 (+) 1581 WP_187597859.1 RNA polymerase factor sigma-54 Machinery gene
  H9K76_RS01560 (H9K76_01560) - 325596..326036 (+) 441 WP_187597860.1 thioesterase family protein -
  H9K76_RS01565 (H9K76_01565) - 326294..329851 (+) 3558 WP_187600402.1 IPTL-CTERM sorting domain-containing protein -

Sequence

Protein


Download         Length: 526 a.a.        Molecular weight: 57324.27 Da        Isoelectric Point: 4.6319

>NTDB_id=477967 H9K76_RS01555 WP_187597859.1 323574..325154(+) (rpoN/rpoN1) [Diaphorobacter ruginosibacter strain DSM 27467]
MKPGLSLRVSQHLALTPQLQQSIKLLQLSTLELDQEVEQMLDDNPFLERETDTATREEFGLDSADTPASGEEAEFSLHAA
GSTTASAGADEPGSAADADPPAAQTESVDWDGDGTVDVQPDDGEWGGDAPARTHTSGSGEDEVDAIDLARAHESLTDHLH
RQALSLHLSEIDSAAVRFLIESLNDDGYLEESLAELAIALAGNDAPTEELEELVHRFTVALRLLQSLEPAGVGARNLGEC
LTLQLHGMNQDEEGDPAIIAVALDICRDSLDLLARRDVKRLTQACGAGEENTRAAMNLIARLEPRPGRRFADVQRQIIVP
DVIVKNVGRGAQMRFTVQLNADVMPRLRVHDVYANALRGQRGNEAHAGLQQRLQEARWFIKNVQQRFDTILRVSTAIVER
QKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMSTPQGTFELKYFFGSGLGTETGGSASSTAVKALIKQLLSS
ENPKKPLSDSKIADMLAEQGIECARRTVAKYREALKIPPANLRKAV

Nucleotide


Download         Length: 1581 bp        

>NTDB_id=477967 H9K76_RS01555 WP_187597859.1 323574..325154(+) (rpoN/rpoN1) [Diaphorobacter ruginosibacter strain DSM 27467]
ATGAAACCGGGACTGTCGCTCCGCGTATCGCAACATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCAAGCTGCTGCA
GCTCTCCACGCTGGAGCTCGACCAGGAGGTCGAGCAGATGCTGGATGACAACCCGTTCCTGGAGCGCGAAACCGATACCG
CCACCCGCGAGGAGTTCGGGCTCGACAGCGCGGACACGCCGGCAAGCGGCGAAGAGGCGGAGTTCTCCCTGCATGCCGCA
GGCTCCACCACGGCCTCGGCAGGCGCCGACGAGCCAGGTTCCGCAGCCGATGCAGACCCGCCGGCGGCCCAGACCGAGAG
CGTCGACTGGGATGGCGACGGCACGGTCGATGTCCAGCCGGATGATGGCGAGTGGGGGGGCGATGCTCCCGCGCGCACCC
ATACGAGCGGGTCCGGCGAGGATGAAGTGGACGCCATCGATCTTGCGCGTGCACATGAGTCGCTCACCGACCACCTGCAC
CGGCAGGCACTCTCGCTGCATCTGTCGGAGATCGACAGTGCCGCCGTGCGCTTCCTAATTGAATCGCTGAACGACGATGG
CTACCTGGAGGAATCGCTTGCCGAACTGGCGATTGCCCTGGCAGGCAACGATGCGCCCACCGAGGAGCTCGAAGAGCTCG
TGCATCGCTTCACCGTGGCGCTGCGCCTGCTGCAGAGCCTCGAGCCAGCGGGCGTCGGCGCGCGCAACCTGGGTGAATGC
CTCACGCTGCAGTTGCACGGCATGAACCAGGACGAGGAGGGCGATCCGGCGATCATCGCCGTGGCGCTCGACATCTGCCG
CGATTCGCTGGACCTGCTGGCGCGCCGCGACGTCAAGCGCCTCACGCAGGCCTGTGGCGCCGGTGAGGAGAACACGCGCG
CGGCCATGAACCTGATTGCGCGCCTGGAACCTCGCCCGGGGCGGCGCTTTGCCGATGTCCAGCGCCAGATCATCGTGCCC
GACGTGATCGTCAAGAACGTGGGGCGCGGCGCGCAGATGCGGTTCACGGTGCAGCTCAACGCGGATGTGATGCCGCGCCT
GCGCGTGCATGACGTCTACGCCAACGCCCTGCGCGGCCAGCGCGGCAACGAGGCCCATGCGGGCCTGCAGCAGCGGCTGC
AGGAGGCGCGCTGGTTCATCAAGAACGTGCAGCAGCGCTTCGACACCATCCTGCGCGTGTCCACGGCCATCGTCGAGCGC
CAGAAGAATTTCTTCGTGCACGGCGAACTGGCCATGCGTCCACTGGTGCTGCGCGACATCGCGGACGAGCTGGGCCTGCA
TGAGTCCACCATCAGCCGCGTGACGACGGCCAAGTACATGTCCACGCCCCAGGGGACCTTCGAGCTCAAGTACTTCTTCG
GATCGGGCCTGGGAACCGAGACGGGCGGCAGCGCGTCGAGCACGGCCGTGAAGGCGTTGATCAAGCAGCTGCTGAGCTCC
GAAAACCCGAAGAAGCCGCTGTCGGACAGCAAGATCGCGGACATGCTGGCGGAGCAGGGCATCGAGTGCGCCCGCCGCAC
CGTGGCCAAGTACCGCGAGGCGCTGAAGATTCCCCCGGCCAATCTGCGCAAGGCGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(148-353)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(369-524)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G9RPT6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.542

100

0.53