Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J1G54_RS09010 Genome accession   NZ_CP071491
Coordinates   1778166..1779551 (-) Length   461 a.a.
NCBI ID   WP_203399225.1    Uniprot ID   -
Organism   Glaesserella parasuis strain GHP1807     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1773166..1784551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1G54_RS08985 (J1G54_08985) clpP 1773183..1773764 (+) 582 WP_005711766.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  J1G54_RS08990 (J1G54_08990) clpX 1773771..1775021 (+) 1251 WP_203399223.1 ATP-dependent protease ATP-binding subunit ClpX Regulator
  J1G54_RS08995 (J1G54_08995) dapE 1775095..1776228 (-) 1134 WP_160436358.1 succinyl-diaminopimelate desuccinylase -
  J1G54_RS09000 (J1G54_09000) - 1776312..1776980 (-) 669 WP_035491631.1 prepilin peptidase -
  J1G54_RS09005 (J1G54_09005) pilC 1776977..1778173 (-) 1197 WP_207342679.1 type II secretion system F family protein Machinery gene
  J1G54_RS09010 (J1G54_09010) pilB 1778166..1779551 (-) 1386 WP_203399225.1 GspE/PulE family protein Machinery gene
  J1G54_RS09015 (J1G54_09015) pilA 1779599..1780057 (-) 459 WP_203399226.1 pilin Machinery gene
  J1G54_RS09020 (J1G54_09020) radA/sms 1780245..1781621 (+) 1377 WP_005711784.1 DNA repair protein RadA Machinery gene
  J1G54_RS09025 (J1G54_09025) gmk 1781674..1782300 (-) 627 WP_203399227.1 guanylate kinase -
  J1G54_RS09030 (J1G54_09030) gpt 1782538..1783017 (-) 480 WP_005714020.1 xanthine phosphoribosyltransferase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52666.35 Da        Isoelectric Point: 4.9784

>NTDB_id=477347 J1G54_RS09010 WP_203399225.1 1778166..1779551(-) (pilB) [Glaesserella parasuis strain GHP1807]
MSQYTVCEQQTERIFDISPSRWQQNCEEKELLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPLYEESELAFAEQEQEQLNLSDPIIQLLDNLFKFCLTQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEDSAKMALRASQTGHLVLSTLHTNDALSAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGTGC
DECYQGYKGRIGIYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNEIEVDRVLGDE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=477347 J1G54_RS09010 WP_203399225.1 1778166..1779551(-) (pilB) [Glaesserella parasuis strain GHP1807]
ATGTCCCAATACACCGTCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAACAAAATTGCGA
AGAAAAAGAGCTGTTATTGCGTTATCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAACCTGTGGTAATTGCTTCTGATGAG
CTTAAATACCTGCTTAACGCACTCTCGCCTGAGCAACAACCCCTTTATGAAGAGAGCGAACTGGCTTTCGCAGAACAAGA
GCAAGAACAGCTCAACTTAAGCGATCCGATTATTCAACTGCTCGATAATCTTTTTAAATTCTGTTTAACCCAAAATGCGT
CTGATATTCATATTGAACCACGCAAGCAAAAATTGATTATCCGCTTACGCATTGACGGTGTTTTGCACCTGTATAAAAGC
CTGTCTATTCAACTGGCAAGCCGACTTATTTCACGGATAAAACTGCTGGCGAAGCTAGATATTAGTGAATTACGCCAGCC
ACAAGATGGGCAATTTAGCTTTACTACCGCCTTGGCGGACACCCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GCGAAAAACTGGTATTGCGCCTACAAAAAAACAAACCAACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCTTGTATAGTGCCTTAAACTACCTCAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCTGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTATTACGCACCTTTCTACGCCAA
GATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGATAGTGCAAAAATGGCATTAAGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTATCAGCGGTCGAACGTTTGTTACAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTCATTGCACAACGCCTTGTGCGTAAGCTCTGCAAAAAGTGTCGTGGCACAGGCTGT
GATGAGTGCTATCAAGGCTATAAAGGGCGGATCGGGATTTACCAATGTTTAAGTAGAACTGCAAAAAAATTCGATAAACA
GACCGCTTACTTAGATTTTTCCAGCTTACGAGAGAGTGCTAAACAAAAAATTGAACAACAACTAACCAACGAAATCGAAG
TGGATAGGGTATTAGGCGATGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

96.746

100

0.967
  pilB Haemophilus influenzae Rd KW20

57.363

98.698

0.566
  pilB Haemophilus influenzae 86-028NP

56.739

99.783

0.566
  pilB Vibrio cholerae strain A1552

38.554

100

0.416
  pilB Vibrio parahaemolyticus RIMD 2210633

39.709

100

0.414
  pilB Vibrio campbellii strain DS40M4

39.709

100

0.414
  pilB Acinetobacter baylyi ADP1

36.436

100

0.399
  pilB Acinetobacter baumannii D1279779

35.798

100

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.085

100

0.388
  pilB Legionella pneumophila strain ERS1305867

40.455

95.445

0.386
  pilF Neisseria gonorrhoeae MS11

36.632

100

0.377