Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J1F34_RS12040 Genome accession   NZ_CP071441
Coordinates   2499080..2499547 (+) Length   155 a.a.
NCBI ID   WP_000340171.1    Uniprot ID   -
Organism   Escherichia coli strain EcPF20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2494080..2504547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1F34_RS11995 (J1F34_11995) rplP 2494190..2494600 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  J1F34_RS12000 (J1F34_12000) rpsC 2494613..2495314 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  J1F34_RS12005 (J1F34_12005) rplV 2495332..2495664 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  J1F34_RS12010 (J1F34_12010) rpsS 2495679..2495957 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  J1F34_RS12015 (J1F34_12015) rplB 2495974..2496795 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  J1F34_RS12020 (J1F34_12020) rplW 2496813..2497115 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  J1F34_RS12025 (J1F34_12025) rplD 2497112..2497717 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  J1F34_RS12030 (J1F34_12030) rplC 2497728..2498357 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  J1F34_RS12035 (J1F34_12035) rpsJ 2498390..2498701 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  J1F34_RS12040 (J1F34_12040) pilD 2499080..2499547 (+) 468 WP_000340171.1 A24 family peptidase Machinery gene
  J1F34_RS12045 (J1F34_12045) bfr 2499544..2500020 (-) 477 WP_000675515.1 bacterioferritin -
  J1F34_RS12050 (J1F34_12050) bfd 2500093..2500287 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  J1F34_RS12055 (J1F34_12055) tuf 2500470..2501654 (-) 1185 WP_000031783.1 elongation factor Tu -
  J1F34_RS12060 (J1F34_12060) fusA 2501725..2503839 (-) 2115 WP_000124700.1 elongation factor G -
  J1F34_RS12065 (J1F34_12065) rpsG 2503936..2504406 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16938.08 Da        Isoelectric Point: 8.1296

>NTDB_id=477003 J1F34_RS12040 WP_000340171.1 2499080..2499547(+) (pilD) [Escherichia coli strain EcPF20]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=477003 J1F34_RS12040 WP_000340171.1 2499080..2499547(+) (pilD) [Escherichia coli strain EcPF20]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361
  pilD Neisseria gonorrhoeae MS11

38.095

94.839

0.361