Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   J1F19_RS04370 Genome accession   NZ_CP071439
Coordinates   877155..878582 (+) Length   475 a.a.
NCBI ID   WP_001546874.1    Uniprot ID   -
Organism   Escherichia coli strain EcPNK004     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 872155..883582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1F19_RS04330 (J1F19_04330) yeeW 872339..872533 (+) 195 WP_000988600.1 DUF5983 family protein -
  J1F19_RS04335 (J1F19_04335) - 872579..872659 (+) 81 Protein_867 hypothetical protein -
  J1F19_RS04340 (J1F19_04340) - 872948..873076 (-) 129 Protein_868 transposase domain-containing protein -
  J1F19_RS04345 (J1F19_04345) - 873148..873330 (+) 183 WP_032140263.1 EutP/PduV family microcompartment system protein -
  J1F19_RS04350 (J1F19_04350) tmaR 873431..873760 (-) 330 WP_000450409.1 DUF496 family protein -
  J1F19_RS04355 (J1F19_04355) yeeA 873932..874990 (-) 1059 WP_001200891.1 FUSC family protein -
  J1F19_RS04360 (J1F19_04360) sbmC 875188..875661 (-) 474 WP_001105389.1 DNA gyrase inhibitor SbmC -
  J1F19_RS04365 (J1F19_04365) dacD 875780..876946 (-) 1167 WP_000830150.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  J1F19_RS04370 (J1F19_04370) sbcB 877155..878582 (+) 1428 WP_001546874.1 exodeoxyribonuclease I Machinery gene
  J1F19_RS04375 (J1F19_04375) tsuB 878625..878852 (-) 228 WP_001546876.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  J1F19_RS04380 (J1F19_04380) tsuA 878866..879924 (-) 1059 WP_000492324.1 thiosulfate utilization transporter TsuA/YeeE -
  J1F19_RS04385 (J1F19_04385) plaP 880103..881461 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  J1F19_RS04390 (J1F19_04390) yoeI 881451..881513 (-) 63 WP_010723108.1 membrane protein YoeI -
  J1F19_RS04395 (J1F19_04395) yeeY 881728..882657 (-) 930 WP_019842103.1 LysR substrate-binding domain-containing protein -
  J1F19_RS04400 (J1F19_04400) yeeZ 882703..883527 (-) 825 WP_000754737.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54325.58 Da        Isoelectric Point: 5.3435

>NTDB_id=476895 J1F19_RS04370 WP_001546874.1 877155..878582(+) (sbcB) [Escherichia coli strain EcPNK004]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWS
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPELLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=476895 J1F19_RS04370 WP_001546874.1 877155..878582(+) (sbcB) [Escherichia coli strain EcPNK004]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCTCGGGCGAAAGGAGAAAAC
GAAGCCGCATTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTACGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGAGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCAGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAGCTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTATTGCAGGGTTATGCAGATGAATTGCAGATGCTGGCACAACAAT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581