Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   J0O73_RS03510 Genome accession   NZ_CP071375
Coordinates   728354..729736 (+) Length   460 a.a.
NCBI ID   WP_096220326.1    Uniprot ID   -
Organism   Escherichia coli strain E105     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 723354..734736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0O73_RS03485 (J0O73_03485) deoD 724368..725087 (+) 720 WP_000224877.1 purine-nucleoside phosphorylase -
  J0O73_RS03490 (J0O73_03490) - 725248..725511 (-) 264 WP_000566150.1 helix-turn-helix transcriptional regulator -
  J0O73_RS03495 (J0O73_03495) lplA 725543..726559 (-) 1017 WP_021557509.1 lipoate--protein ligase LplA -
  J0O73_RS03500 (J0O73_03500) ytjB 726587..727231 (-) 645 WP_000124615.1 YtjB family periplasmic protein -
  J0O73_RS03505 (J0O73_03505) serB 727337..728305 (+) 969 WP_001132955.1 phosphoserine phosphatase -
  J0O73_RS03510 (J0O73_03510) radA/sms 728354..729736 (+) 1383 WP_096220326.1 DNA repair protein RadA Machinery gene
  J0O73_RS03515 (J0O73_03515) nadR 729757..730989 (+) 1233 WP_053883158.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  J0O73_RS27145 (J0O73_03520) - 731261..731374 (-) 114 Protein_680 hypothetical protein -
  J0O73_RS03525 (J0O73_03525) - 731456..731668 (-) 213 WP_000848792.1 DUF1471 domain-containing protein -
  J0O73_RS03530 (J0O73_03530) - 731826..732242 (-) 417 WP_053883157.1 SRPBCC family protein -
  J0O73_RS03535 (J0O73_03535) - 732268..733065 (-) 798 WP_000762716.1 SDR family oxidoreductase -
  J0O73_RS03540 (J0O73_03540) - 733085..734095 (-) 1011 WP_096220325.1 aldo/keto reductase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49456.04 Da        Isoelectric Point: 7.1936

>NTDB_id=476748 J0O73_RS03510 WP_096220326.1 728354..729736(+) (radA/sms) [Escherichia coli strain E105]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGIFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=476748 J0O73_RS03510 WP_096220326.1 728354..729736(+) (radA/sms) [Escherichia coli strain E105]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTATTAGGCGGCGGGGTAGTGCCGGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGG
GAAATCCACGCTGTTGCTACAAACGCTGTGCAAACTGGCGCAGCAGATGAAAACGCTGTATGTCACTGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGTCTCGGCCTGCCGACTGATAATCTCAATATGTTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
TCAGTCATCGCCAGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCATCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATCTTTTTAAGCCGCGGCGATGAAG
TGACCTCTGGTAGTTCAGTGATGGTGGTATGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCGCGCCGCGTGGCGGTAGGTCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTATTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCG
ATTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

47.046

99.348

0.467
  radA Streptococcus mitis SK321

44.079

99.13

0.437
  radA Streptococcus pneumoniae D39

43.64

99.13

0.433
  radA Streptococcus pneumoniae TIGR4

43.64

99.13

0.433
  radA Streptococcus mitis NCTC 12261

43.64

99.13

0.433
  radA Streptococcus pneumoniae R6

43.64

99.13

0.433
  radA Streptococcus pneumoniae Rx1

43.64

99.13

0.433