Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J0G23_RS12225 Genome accession   NZ_CP071263
Coordinates   2468703..2469380 (+) Length   225 a.a.
NCBI ID   WP_001241108.1    Uniprot ID   -
Organism   Escherichia coli strain MEI003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2463703..2474380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0G23_RS12190 (J0G23_12115) gspG 2464183..2464620 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  J0G23_RS12195 (J0G23_12120) gspH 2464628..2465137 (+) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  J0G23_RS12200 (J0G23_12125) gspI 2465134..2465511 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  J0G23_RS12205 (J0G23_12130) gspJ 2465504..2466091 (+) 588 WP_000610635.1 type II secretion system minor pseudopilin GspJ -
  J0G23_RS12210 (J0G23_12135) gspK 2466084..2467067 (+) 984 WP_001058524.1 type II secretion system minor pseudopilin GspK -
  J0G23_RS12215 (J0G23_12140) gspL 2467082..2468245 (+) 1164 WP_001115242.1 type II secretion system protein GspL -
  J0G23_RS12220 (J0G23_12145) gspM 2468242..2468703 (+) 462 WP_024230504.1 type II secretion system protein GspM -
  J0G23_RS12225 (J0G23_12150) pilD 2468703..2469380 (+) 678 WP_001241108.1 prepilin peptidase GspO Machinery gene
  J0G23_RS12230 (J0G23_12155) bfr 2469409..2469885 (-) 477 WP_000675504.1 bacterioferritin -
  J0G23_RS12235 (J0G23_12160) bfd 2469957..2470151 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  J0G23_RS12240 (J0G23_12165) chiA 2470320..2473022 (-) 2703 WP_024230503.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24836.73 Da        Isoelectric Point: 7.9462

>NTDB_id=476442 J0G23_RS12225 WP_001241108.1 2468703..2469380(+) (pilD) [Escherichia coli strain MEI003]
MAMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFRQKKYAWNDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMISLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=476442 J0G23_RS12225 WP_001241108.1 2468703..2469380(+) (pilD) [Escherichia coli strain MEI003]
ATGGCAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCCGGCAAAAAAAATATGCCTGGAATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTGCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAGCCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378