Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   J0G24_RS20780 Genome accession   NZ_CP071259
Coordinates   4283679..4285061 (-) Length   460 a.a.
NCBI ID   WP_001029690.1    Uniprot ID   -
Organism   Escherichia coli strain MEI005     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4278679..4290061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0G24_RS20750 (J0G24_20555) - 4279326..4280336 (+) 1011 WP_000023619.1 aldo/keto reductase -
  J0G24_RS20755 (J0G24_20560) - 4280356..4281153 (+) 798 WP_000762718.1 SDR family oxidoreductase -
  J0G24_RS20760 (J0G24_20565) - 4281179..4281595 (+) 417 WP_001140838.1 SRPBCC family protein -
  J0G24_RS20765 (J0G24_20570) - 4281753..4281965 (+) 213 WP_000848790.1 DUF1471 domain-containing protein -
  J0G24_RS20770 - 4282038..4282154 (+) 117 Protein_4083 hypothetical protein -
  J0G24_RS20775 (J0G24_20580) nadR 4282426..4283658 (-) 1233 WP_000093813.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  J0G24_RS20780 (J0G24_20585) radA/sms 4283679..4285061 (-) 1383 WP_001029690.1 DNA repair protein RadA Machinery gene
  J0G24_RS20785 (J0G24_20590) serB 4285110..4286078 (-) 969 WP_001132972.1 phosphoserine phosphatase -
  J0G24_RS20790 (J0G24_20595) ytjB 4286184..4286828 (+) 645 WP_000124607.1 YtjB family periplasmic protein -
  J0G24_RS20795 (J0G24_20600) lplA 4286856..4287872 (+) 1017 WP_000105867.1 lipoate--protein ligase LplA -
  J0G24_RS20800 (J0G24_20605) - 4287904..4288167 (+) 264 WP_000566145.1 helix-turn-helix transcriptional regulator -
  J0G24_RS20805 (J0G24_20610) deoD 4288328..4289047 (-) 720 WP_000224879.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49458.06 Da        Isoelectric Point: 7.1936

>NTDB_id=476384 J0G24_RS20780 WP_001029690.1 4283679..4285061(-) (radA/sms) [Escherichia coli strain MEI005]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLLTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=476384 J0G24_RS20780 WP_001029690.1 4283679..4285061(-) (radA/sms) [Escherichia coli strain MEI005]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGCGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCAGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTCAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCGGGAAGTGCCATTCTGATTGGCGGTAACCCAGGTGCCGG
GAAATCCACATTGCTGTTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCTGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATCCAGGTGATGCATATGGCGGATGT
TCAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGCGTGG
CGATTGTCATGGTTGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTCTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCTGCCATCTTTTTAAGCCGCGGTGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATCCAGGCGCTGGTCGATCAC
TCGATGATGGCGAACCCACGCCGTGTGGCAGTGGGGCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCGGTGCTGCACCG
TCACGGTGGGTTGCAAATGGCCGATCAGGATGTATTTGTTAACGTGGTCGGCGGCGTGAAGGTGACGGAAACCAGTGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGCCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGGCGGGC
GATTGTTCCGGCGGCTAATGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

AAA domain (AAA_25)

(89-225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis NCTC 12261

43.86

99.13

0.435
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43