Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   H8N04_RS06735 Genome accession   NZ_CP060491
Coordinates   1316014..1316988 (+) Length   324 a.a.
NCBI ID   WP_199944275.1    Uniprot ID   -
Organism   Staphylococcus aureus strain WH39     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1311014..1321988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8N04_RS06700 (H8N04_06705) rpmG 1311474..1311623 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  H8N04_RS06705 (H8N04_06710) - 1311832..1312371 (+) 540 WP_000164545.1 5-formyltetrahydrofolate cyclo-ligase -
  H8N04_RS06710 (H8N04_06715) - 1312383..1313846 (+) 1464 WP_001017863.1 rhomboid family protein -
  H8N04_RS06715 (H8N04_06720) - 1313827..1314030 (+) 204 WP_000087561.1 YqgQ family protein -
  H8N04_RS06720 (H8N04_06725) - 1314027..1315013 (+) 987 WP_000161314.1 ROK family glucokinase -
  H8N04_RS06725 (H8N04_06730) - 1315013..1315342 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  H8N04_RS06730 (H8N04_06735) - 1315339..1315962 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  H8N04_RS06735 (H8N04_06740) comGA 1316014..1316988 (+) 975 WP_199944275.1 competence type IV pilus ATPase ComGA Machinery gene
  H8N04_RS06740 (H8N04_06745) comGB 1316960..1318030 (+) 1071 Protein_1286 competence type IV pilus assembly protein ComGB -
  H8N04_RS06745 (H8N04_06750) comGC 1318044..1318355 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  H8N04_RS06750 (H8N04_06755) comGD 1318333..1318779 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  H8N04_RS06755 (H8N04_06760) comGE 1318766..1319065 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  H8N04_RS06760 (H8N04_06765) comGF 1318983..1319480 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  H8N04_RS06765 (H8N04_06770) - 1319577..1319723 (+) 147 WP_001789879.1 hypothetical protein -
  H8N04_RS06770 (H8N04_06775) - 1319713..1320237 (+) 525 WP_001015117.1 shikimate kinase -
  H8N04_RS06775 (H8N04_06780) gcvT 1320396..1321487 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36854.98 Da        Isoelectric Point: 9.0399

>NTDB_id=476313 H8N04_RS06735 WP_199944275.1 1316014..1316988(+) (comGA) [Staphylococcus aureus strain WH39]
MKILFQEIINKAIEMKASDVHFISVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
HKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=476313 H8N04_RS06735 WP_199944275.1 1316014..1316988(+) (comGA) [Staphylococcus aureus strain WH39]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTTCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATAAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
CATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

98.765

100

0.988
  comGA Staphylococcus aureus N315

98.765

100

0.988