Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H8S71_RS12650 Genome accession   NZ_CP060417
Coordinates   2474865..2475275 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis subsp. subtilis strain ONU 559     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2469865..2480275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8S71_RS12620 (H8S71_12615) - 2469951..2470091 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  H8S71_RS12625 (H8S71_12620) yqeF 2470397..2471128 (-) 732 WP_085186514.1 SGNH/GDSL hydrolase family protein -
  H8S71_RS12630 (H8S71_12625) cwlH 2471380..2472132 (-) 753 WP_085186516.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  H8S71_RS12635 (H8S71_12630) yqeD 2472319..2472945 (+) 627 WP_172861316.1 TVP38/TMEM64 family protein -
  H8S71_RS12640 (H8S71_12635) gnd 2472965..2473858 (-) 894 WP_046160611.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  H8S71_RS12645 (H8S71_12640) yqeB 2474110..2474832 (+) 723 WP_021480070.1 hypothetical protein -
  H8S71_RS12650 (H8S71_12645) nucA/comI 2474865..2475275 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  H8S71_RS12655 (H8S71_12650) sigK 2475471..2476199 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  H8S71_RS12660 (H8S71_12655) - 2476199..2476315 (+) 117 WP_070547560.1 hypothetical protein -
  H8S71_RS12665 (H8S71_12660) - 2476281..2476478 (-) 198 Protein_2460 recombinase family protein -
  H8S71_RS20530 - 2476436..2476587 (-) 152 Protein_2461 hypothetical protein -
  H8S71_RS12670 (H8S71_12665) psiE 2477131..2477547 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  H8S71_RS12675 (H8S71_12670) yrkQ 2477593..2478891 (-) 1299 WP_187001807.1 two-component system sensor histidine kinase YrkQ -
  H8S71_RS12680 (H8S71_12675) yrkP 2478878..2479573 (-) 696 WP_021480140.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=475904 H8S71_RS12650 WP_009967785.1 2474865..2475275(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain ONU 559]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=475904 H8S71_RS12650 WP_009967785.1 2474865..2475275(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain ONU 559]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529