Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   H8S71_RS12400 Genome accession   NZ_CP060417
Coordinates   2431424..2432191 (-) Length   255 a.a.
NCBI ID   WP_015384118.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain ONU 559     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2426424..2437191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8S71_RS12380 (H8S71_12375) ppsR 2426598..2427410 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  H8S71_RS12385 (H8S71_12380) ccpN 2427436..2428074 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  H8S71_RS12390 (H8S71_12385) glyS 2428207..2430246 (-) 2040 WP_169037808.1 glycine--tRNA ligase subunit beta -
  H8S71_RS12395 (H8S71_12390) glyQ 2430239..2431126 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  H8S71_RS12400 (H8S71_12395) recO 2431424..2432191 (-) 768 WP_015384118.1 DNA repair protein RecO Machinery gene
  H8S71_RS12405 (H8S71_12400) yqzL 2432228..2432371 (-) 144 WP_003230053.1 YqzL family protein -
  H8S71_RS12410 (H8S71_12405) era 2432519..2433424 (-) 906 WP_024572411.1 GTPase Era -
  H8S71_RS12415 (H8S71_12410) - 2433405..2433815 (-) 411 WP_003230049.1 cytidine deaminase -
  H8S71_RS12420 (H8S71_12415) unpK 2433934..2434305 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  H8S71_RS12425 (H8S71_12420) ybeY 2434286..2434759 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  H8S71_RS12430 (H8S71_12425) pgpH 2434760..2436895 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29332.01 Da        Isoelectric Point: 8.3080

>NTDB_id=475900 H8S71_RS12400 WP_015384118.1 2431424..2432191(-) (recO) [Bacillus subtilis subsp. subtilis strain ONU 559]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKSNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=475900 H8S71_RS12400 WP_015384118.1 2431424..2432191(-) (recO) [Bacillus subtilis subsp. subtilis strain ONU 559]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGTC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTTTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTCTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992