Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H8S71_RS01985 Genome accession   NZ_CP060417
Coordinates   372059..372508 (-) Length   149 a.a.
NCBI ID   WP_070548532.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain ONU 559     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 367059..377508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8S71_RS01945 (H8S71_01940) - 367380..367577 (+) 198 WP_122060560.1 hypothetical protein -
  H8S71_RS01950 (H8S71_01945) yckC 367597..368052 (+) 456 WP_049140212.1 RDD family protein -
  H8S71_RS01955 (H8S71_01950) yckD 368133..368465 (+) 333 WP_032724908.1 YckD family protein -
  H8S71_RS01960 (H8S71_01955) - 368593..369015 (+) 423 WP_170939093.1 MarR family transcriptional regulator -
  H8S71_RS01965 (H8S71_01960) - 369003..369344 (+) 342 WP_032724911.1 DUF3147 family protein -
  H8S71_RS01970 (H8S71_01965) - 369363..370091 (+) 729 WP_021481826.1 AAA family ATPase -
  H8S71_RS01975 (H8S71_01970) bglC 370164..371597 (+) 1434 WP_072175231.1 6-phospho-beta-glucosidase -
  H8S71_RS01980 (H8S71_01975) nin/comJ 371634..372032 (-) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  H8S71_RS01985 (H8S71_01980) nucA/comI 372059..372508 (-) 450 WP_070548532.1 DNA-entry nuclease Machinery gene
  H8S71_RS01990 (H8S71_01985) tlpC 372676..374397 (-) 1722 WP_169037974.1 methyl-accepting chemotaxis protein TlpC -
  H8S71_RS01995 (H8S71_01990) hxlB 374508..375065 (-) 558 WP_041850679.1 6-phospho-3-hexuloisomerase -
  H8S71_RS02000 (H8S71_01995) hxlA 375071..375703 (-) 633 WP_032724921.1 3-hexulose-6-phosphate synthase -
  H8S71_RS02005 (H8S71_02000) hxlR 375935..376297 (+) 363 WP_187002048.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.35 Da        Isoelectric Point: 4.4397

>NTDB_id=475859 H8S71_RS01985 WP_070548532.1 372059..372508(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain ONU 559]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLQDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=475859 H8S71_RS01985 WP_070548532.1 372059..372508(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain ONU 559]
GTGAACATCACGACGGACATCATAAAAACAATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTACAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98