Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   H8N02_RS02705 Genome accession   NZ_CP060413
Coordinates   584276..585865 (+) Length   529 a.a.
NCBI ID   WP_210607095.1    Uniprot ID   -
Organism   Comamonas kerstersii strain 3132976     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 579276..590865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8N02_RS02680 (H8N02_02680) argS 579621..581327 (+) 1707 WP_054065349.1 arginine--tRNA ligase -
  H8N02_RS02685 (H8N02_02685) - 581370..582008 (+) 639 WP_058879929.1 SPOR domain-containing protein -
  H8N02_RS02690 (H8N02_02690) - 582064..582708 (+) 645 WP_054065351.1 thiol:disulfide interchange protein DsbA/DsbL -
  H8N02_RS02695 (H8N02_02695) lptA 582810..583445 (+) 636 WP_058880045.1 lipopolysaccharide transport periplasmic protein LptA -
  H8N02_RS02700 (H8N02_02700) lptB 583445..584215 (+) 771 WP_054065352.1 LPS export ABC transporter ATP-binding protein -
  H8N02_RS02705 (H8N02_02705) rpoN/rpoN1 584276..585865 (+) 1590 WP_210607095.1 RNA polymerase factor sigma-54 Machinery gene
  H8N02_RS02710 (H8N02_02710) - 586040..586984 (+) 945 WP_054065354.1 sugar ABC transporter substrate-binding protein -
  H8N02_RS02715 (H8N02_02715) - 586994..588565 (+) 1572 WP_151045622.1 sugar ABC transporter ATP-binding protein -
  H8N02_RS02720 (H8N02_02720) - 588583..589566 (+) 984 WP_058879933.1 ABC transporter permease -
  H8N02_RS02725 (H8N02_02725) rbsK 589578..590489 (+) 912 WP_210607096.1 ribokinase -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58758.56 Da        Isoelectric Point: 4.9801

>NTDB_id=475800 H8N02_RS02705 WP_210607095.1 584276..585865(+) (rpoN/rpoN1) [Comamonas kerstersii strain 3132976]
MKPSLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLELSAEEVEREAFGVEHANSAPEADVHASDETYF
SVADSAVSTGAEGQKDSEIPVAEAADLPQDWEGDGSVDMGALDDAEWGGEAPARGKRNEDEDAPSALELARCHESLTDHL
HQQALSLRLSEVQRCALRFLIENLNDDGYLEDSLQELAIGLAGSEDEEQLEELMHHFTVALRLLQSLEPVGVGARDLAEC
LRLQLRYLQEQGGDDPALLEAALQLCAQPLDMLARRDVRKLMQACGLPESRVRAALALIARLEPKPGRRFVDVERHVIVP
DVIVQKTNRKGGDGLMQFSVQLNHDVMPRLRVHEVYAGALRSHKGSEGHAMLQQRLQEARWFIKNVQQRFDTILRVSKAI
VERQKNFFIHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPHGTYELKFFFGSGLGTETGGNASSTAVRALIRQF
VSAENPKKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=475800 H8N02_RS02705 WP_210607095.1 584276..585865(+) (rpoN/rpoN1) [Comamonas kerstersii strain 3132976]
TTGAAGCCAAGTTTGTCACTGCGGGTCTCACAACATCTGGCGTTGACGCCGCAACTTCAACAATCTATTCGACTGCTGCA
GCTGTCGACCCTGGAGCTTTCTCAGGAAGTCGAGCAGATGCTCGACGAAAACCCATTTCTTGAATTGTCTGCCGAAGAGG
TCGAACGCGAGGCCTTTGGCGTGGAGCATGCAAATTCCGCGCCAGAGGCGGATGTGCATGCCAGCGACGAAACTTATTTT
TCTGTAGCTGACAGCGCCGTATCCACGGGCGCTGAAGGGCAAAAAGATTCAGAAATTCCCGTGGCAGAAGCTGCCGATCT
GCCCCAGGACTGGGAGGGCGATGGCAGTGTAGACATGGGGGCGTTGGATGACGCAGAGTGGGGTGGTGAAGCACCGGCAC
GCGGCAAGCGCAATGAGGACGAGGATGCCCCTTCTGCACTGGAACTGGCCCGCTGCCACGAATCTCTGACGGACCATCTG
CACCAGCAGGCACTGTCGCTGCGCCTGTCGGAAGTGCAGCGCTGCGCCCTGCGCTTTCTGATTGAAAACCTCAACGACGA
CGGCTATCTGGAAGACTCGCTGCAAGAGCTGGCTATTGGCCTGGCAGGCAGTGAAGATGAAGAACAGCTTGAGGAGCTGA
TGCACCACTTCACGGTGGCGCTGCGTCTGCTGCAGTCGCTGGAACCCGTGGGTGTGGGGGCGCGTGATCTGGCTGAATGC
CTGCGCCTGCAGCTGCGCTATCTGCAGGAGCAAGGCGGTGACGACCCTGCGCTGCTGGAAGCCGCCTTGCAGCTGTGTGC
CCAGCCGCTGGACATGCTGGCGCGCCGTGATGTGCGCAAGCTCATGCAGGCCTGTGGTCTGCCTGAAAGCCGCGTGCGCG
CAGCGCTGGCGCTGATTGCACGGCTGGAGCCCAAGCCCGGGCGGCGCTTTGTGGATGTGGAGCGGCATGTGATCGTGCCC
GATGTGATCGTGCAGAAAACGAACCGCAAAGGGGGCGATGGCCTGATGCAGTTCAGCGTGCAGCTCAACCACGATGTGAT
GCCGCGTCTGCGTGTGCACGAGGTCTATGCCGGTGCGCTGCGCAGCCACAAGGGCAGCGAGGGCCACGCCATGCTGCAGC
AGCGCCTGCAGGAGGCGCGCTGGTTCATCAAGAACGTGCAGCAGCGCTTTGACACCATCTTGCGCGTGTCCAAAGCCATT
GTGGAGCGGCAGAAAAACTTCTTCATCCACGGTGAGCTGGCCATGCGTCCGCTGGTGCTGCGCGATATTGCGGACGAGCT
GGGGCTGCACGAATCCACCATCAGCCGTGTGACGACGGCCAAGTACATGGCCACGCCCCACGGCACCTATGAGTTGAAGT
TCTTCTTCGGTTCCGGCCTGGGTACAGAGACGGGCGGCAATGCGTCGAGTACGGCAGTGCGTGCGCTGATCCGCCAGTTT
GTGAGTGCAGAGAACCCCAAAAAACCGCTGTCGGACAGCCAGATTGCCGAGATGCTCAAGGAGCAGGGCATCGAATGTGC
CCGCCGCACGGTGGCGAAATACCGGGAGGCGCTGAAAATTGCGCCAGCAAATTTGAGAAAAGCGTTGTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(371-527)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-356)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.166

100

0.524