Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   H7F36_RS03485 Genome accession   NZ_CP060296
Coordinates   719293..720906 (-) Length   537 a.a.
NCBI ID   WP_187053364.1    Uniprot ID   A0A7G8W1S3
Organism   Variovorax sp. PAMC28562     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 714293..725906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F36_RS03465 (H7F36_03465) - 715153..715557 (-) 405 WP_187053360.1 putative toxin-antitoxin system toxin component, PIN family -
  H7F36_RS03470 (H7F36_03470) - 715615..716154 (+) 540 WP_187053361.1 cysteine hydrolase family protein -
  H7F36_RS03475 (H7F36_03475) - 716179..717468 (-) 1290 WP_187053362.1 class I SAM-dependent RNA methyltransferase -
  H7F36_RS03480 (H7F36_03480) - 717493..719283 (+) 1791 WP_187053363.1 hybrid sensor histidine kinase/response regulator -
  H7F36_RS03485 (H7F36_03485) rpoN/rpoN1 719293..720906 (-) 1614 WP_187053364.1 RNA polymerase factor sigma-54 Machinery gene
  H7F36_RS03490 (H7F36_03490) lptB 720917..721696 (-) 780 WP_187054784.1 LPS export ABC transporter ATP-binding protein -
  H7F36_RS03495 (H7F36_03495) lptA 721696..722457 (-) 762 WP_187053365.1 lipopolysaccharide transport periplasmic protein LptA -
  H7F36_RS03500 (H7F36_03500) - 722558..723199 (-) 642 WP_187054785.1 thiol:disulfide interchange protein DsbA/DsbL -
  H7F36_RS03505 (H7F36_03505) - 723363..724112 (-) 750 WP_187053366.1 SPOR domain-containing protein -
  H7F36_RS03510 (H7F36_03510) argS 724127..725824 (-) 1698 WP_187053367.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 537 a.a.        Molecular weight: 59164.52 Da        Isoelectric Point: 4.7945

>NTDB_id=475533 H7F36_RS03485 WP_187053364.1 719293..720906(-) (rpoN/rpoN1) [Variovorax sp. PAMC28562]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDDNPFLERTAEEAAREEFGIDVSDAPAPREEHDSDRQSE
SDGEFAPSALATNADGASTTTTASETEAPASEMSDQEPDWGGDGTVEMTPDDGEWGGEAPASSNNLGPNDGSERTDATEL
ARSQESLQSFLHRQALSLRLNENDQAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDGLVHHFQVALGLLQSLEPV
GVGARDLGECLAIQLKALAHENETVEEKQIRKTAILICKQPMELLARRDFKRLATLARSNEEEVRSALQMIAKLEPKPGR
RFVDVERNIVVPDVIVTRTGRGATLKFRVMLNPDVMPRLRVHDIYAGALKAHKGEGSQALSQRLQEARWFIKNIQQRFDT
ILRVSNAIVERQKSFFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMSTPYGTVELKYFFGSALGTETGGNASSTA
VRALIKQLVSAESIKKPLSDSQISDMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1614 bp        

>NTDB_id=475533 H7F36_RS03485 WP_187053364.1 719293..720906(-) (rpoN/rpoN1) [Variovorax sp. PAMC28562]
ATGAAGCAAGGCCTGTCGCTGCGCGTATCGCAGCACCTCGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTTTTGCA
GTTGTCCACGCTCGAGTTGAGCCAGGAAGTCGAGCAGATGCTCGACGACAACCCGTTCCTGGAGCGCACGGCCGAAGAAG
CAGCACGCGAAGAGTTCGGCATCGACGTGTCCGACGCGCCGGCGCCGCGTGAAGAGCACGACAGCGACCGCCAGAGCGAG
AGCGACGGCGAGTTCGCGCCGAGCGCGCTGGCGACCAACGCCGACGGCGCGAGCACCACCACGACCGCCAGCGAGACCGA
AGCGCCGGCCAGCGAGATGTCCGACCAGGAGCCCGACTGGGGCGGCGATGGCACGGTCGAGATGACGCCGGACGATGGCG
AATGGGGCGGCGAGGCGCCCGCCAGCAGCAACAACCTGGGTCCGAACGACGGCAGCGAGCGCACCGACGCCACCGAGCTC
GCGCGCAGCCAGGAGTCGCTGCAATCCTTCCTGCACCGACAGGCGCTGAGCCTGCGGCTGAACGAAAACGACCAGGCCGC
GTTGCGTTTTTTGATCGAATCGTTGAATGACGACGGCTACCTCGAAGACTCGTTGCCAGCGCTGGCATCAGGCCTCGCAG
GCGATGACAACGACCAGTTCGACGGCCTCGTCCACCACTTCCAGGTGGCGCTTGGCCTGTTGCAGAGCCTGGAGCCGGTC
GGCGTCGGCGCACGCGATCTCGGCGAATGCCTGGCGATCCAGCTGAAGGCGCTCGCCCATGAGAACGAGACCGTCGAAGA
AAAGCAGATCCGCAAGACTGCGATCCTGATCTGCAAGCAGCCGATGGAACTGCTCGCGCGGCGCGACTTCAAACGCTTGG
CCACGCTCGCACGCAGCAACGAAGAAGAAGTGCGCAGCGCGCTGCAGATGATTGCCAAGCTGGAGCCCAAGCCGGGTCGC
CGCTTCGTCGATGTCGAACGCAACATCGTGGTGCCCGACGTGATCGTCACCCGCACCGGTCGCGGCGCGACGCTCAAGTT
CCGCGTGATGCTCAACCCCGACGTGATGCCGCGTCTGCGCGTGCACGACATCTACGCCGGCGCGCTCAAGGCGCACAAGG
GCGAGGGCAGCCAGGCGCTGTCGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAACGCTTCGACACC
ATCCTGCGGGTGAGCAACGCCATCGTCGAGCGGCAGAAAAGCTTTTTCGTGCACGGAGAACTCGCCATGCGCCCGTTGGT
GCTGCGTGAGATCGCCGACGAGTTGGGCTTGCACGAGTCGACCATCAGCCGCGTGACCACGGCCAAGTACATGTCGACGC
CTTACGGCACGGTCGAGCTCAAGTACTTCTTCGGCTCGGCACTCGGCACCGAAACCGGCGGCAATGCATCCAGCACGGCA
GTGCGAGCGCTCATCAAGCAACTCGTGAGCGCGGAGAGCATCAAGAAGCCGCTGTCGGACAGCCAGATTTCCGACATGCT
GAAAGAGCAGGGGATCGAATGTGCGCGGCGCACCGTCGCCAAATACCGCGAAGCGCTGCGCATCGCACCGGCGAATCTGC
GCAAGGCCCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(158-365)

Sigma-54, DNA binding domain (Sigma54_DBD)

(379-535)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8W1S3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.727

100

0.508