Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   H7F35_RS14920 Genome accession   NZ_CP060295
Coordinates   3108488..3110086 (+) Length   532 a.a.
NCBI ID   WP_187113603.1    Uniprot ID   A0A7G8VRZ9
Organism   Variovorax sp. PAMC26660     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3103488..3115086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F35_RS14900 (H7F35_14900) - 3105078..3105854 (+) 777 WP_187113599.1 SPOR domain-containing protein -
  H7F35_RS14905 (H7F35_14905) - 3106120..3106767 (+) 648 WP_187113600.1 thiol:disulfide interchange protein DsbA/DsbL -
  H7F35_RS14910 (H7F35_14910) lptA 3106966..3107679 (+) 714 WP_187113601.1 lipopolysaccharide transport periplasmic protein LptA -
  H7F35_RS14915 (H7F35_14915) lptB 3107676..3108470 (+) 795 WP_187113602.1 LPS export ABC transporter ATP-binding protein -
  H7F35_RS14920 (H7F35_14920) rpoN/rpoN1 3108488..3110086 (+) 1599 WP_187113603.1 RNA polymerase factor sigma-54 Machinery gene
  H7F35_RS14925 (H7F35_14925) - 3110097..3111710 (+) 1614 WP_187113604.1 phospholipase D family protein -
  H7F35_RS14930 (H7F35_14930) - 3111724..3112365 (-) 642 WP_187113605.1 LysE family translocator -
  H7F35_RS14935 (H7F35_14935) - 3112500..3112760 (+) 261 WP_187113606.1 hypothetical protein -
  H7F35_RS14940 (H7F35_14940) - 3112790..3113419 (-) 630 WP_187113607.1 YceI family protein -
  H7F35_RS14945 (H7F35_14945) - 3113491..3114078 (-) 588 WP_187113608.1 YceI family protein -
  H7F35_RS14950 (H7F35_14950) - 3114075..3114680 (-) 606 WP_187113609.1 cytochrome b -

Sequence

Protein


Download         Length: 532 a.a.        Molecular weight: 58755.17 Da        Isoelectric Point: 4.7180

>NTDB_id=475525 H7F35_RS14920 WP_187113603.1 3108488..3110086(+) (rpoN/rpoN1) [Variovorax sp. PAMC26660]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAVDTPVQRDEAGEAAESE
FASAPTSSTSTASTETSTPGESDGPAAEIAEREPDWEGDGTVDMAPDDSEWGSDAPARQNNLGNDERADATELARSQESL
QSFLHRQALSLRLNENDCAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDDLVHHFQVALGLLQSLEPVGVGARDL
GECLGIQLRALAAEDRDMDEAQAQVYKIAIAICKQPMELLARRDFKRLATLTRSNEELVRSALQVIARLEPKPGRRFVDV
ERNIVIPDVIVTKTGRGTGIKFRVMLNPEVMPRLRVHDIYAGALKAHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVS
NAIVERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMSTPFGTVELKYFFGSALGTETGGNASSTAVRALI
KQFVTSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1599 bp        

>NTDB_id=475525 H7F35_RS14920 WP_187113603.1 3108488..3110086(+) (rpoN/rpoN1) [Variovorax sp. PAMC26660]
ATGAAGCAAGGGCTGTCCCTTCGCGTCTCGCAGCATCTCGCGCTCACACCCCAGCTGCAGCAGTCGATCCGTCTGCTGCA
GCTCTCCACGCTCGAACTGAGCCAGGAGGTGGAGCAGATGCTCGACGAGAACCCGTTCCTCGAACGCACCGCCGAAGAAG
CGGCGCGTGAGGAGTTCGGGCTCGATGCCGTCGACACGCCGGTCCAGCGCGACGAGGCAGGCGAAGCCGCCGAAAGCGAA
TTCGCCTCGGCGCCCACCAGCAGCACGAGCACCGCGTCCACCGAGACCAGCACCCCGGGCGAATCCGACGGCCCCGCCGC
CGAGATCGCCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGACATGGCACCGGACGACAGCGAGTGGGGCAGCG
ATGCACCCGCGCGACAGAACAACCTGGGCAACGACGAGCGCGCCGACGCCACCGAACTCGCACGCAGCCAGGAGTCGCTG
CAATCGTTCCTGCACCGCCAGGCGCTGAGCCTGCGCCTGAACGAAAACGACTGCGCCGCGCTGCGCTTCCTGATCGAGTC
GCTCAACGACGACGGCTACCTCGAAGACTCGCTGCCTGCCTTGGCTTCGGGCCTCGCGGGCGACGACAACGATCAGTTCG
ACGACCTCGTGCACCACTTTCAGGTGGCGCTGGGCCTGCTGCAGAGCTTGGAGCCCGTGGGCGTGGGCGCGCGCGACCTG
GGCGAATGCCTGGGCATCCAGTTGCGCGCACTGGCTGCCGAAGACCGCGACATGGACGAAGCGCAGGCGCAGGTCTACAA
GATCGCCATTGCCATCTGCAAGCAGCCGATGGAGCTGCTCGCGCGACGCGACTTCAAGCGGCTGGCCACGCTCACCCGCA
GCAACGAGGAGCTGGTGCGCTCGGCGCTGCAGGTCATTGCGCGGCTCGAACCCAAGCCGGGGCGCCGCTTTGTCGACGTC
GAGCGCAACATCGTCATTCCCGACGTGATCGTCACCAAGACCGGACGCGGCACCGGCATCAAGTTTCGCGTCATGCTGAA
CCCCGAGGTGATGCCGCGCCTGCGCGTGCACGACATCTATGCTGGCGCGCTCAAGGCGCACAAGGGCGAGGGCAGCCAGG
CGCTGTCGCAGCGTCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGC
AATGCCATCGTCGAACGGCAAAAGAGCTACTTCGTGCACGGCGAACTCGCGATGCGGCCGCTGGTGCTGCGCGAGATTGC
CGACGAACTGGGGCTGCACGAGTCGACCATCTCGCGCGTGACCACCGCCAAGTACATGTCCACGCCTTTCGGCACGGTCG
AGCTCAAGTATTTCTTCGGCTCTGCGCTGGGCACCGAGACCGGCGGCAACGCATCGAGCACCGCGGTGCGCGCGCTCATC
AAGCAGTTCGTGACCTCCGAGAGCATCAAGAAGCCGCTGTCGGACAGCCAGATCTCCGAGATGCTGAAGGAACAAGGCAT
CGAATGCGCGCGGCGCACGGTGGCCAAGTACCGCGAAGCGCTGCGCATCGCGCCCGCGAACCTGCGCAAAGCCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(374-530)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(151-360)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G8VRZ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.469

100

0.506