Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H7F24_RS14005 Genome accession   NZ_CP060290
Coordinates   2787643..2788023 (+) Length   126 a.a.
NCBI ID   WP_065214465.1    Uniprot ID   -
Organism   Bacillus sp. PAMC22265     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2782643..2793023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F24_RS13995 (H7F24_13995) - 2783140..2786283 (+) 3144 WP_149126620.1 bifunctional cytochrome P450/NADPH--P450 reductase -
  H7F24_RS14000 (H7F24_14000) - 2786477..2787385 (+) 909 WP_024425124.1 trypsin-like serine peptidase -
  H7F24_RS14005 (H7F24_14005) nucA/comI 2787643..2788023 (+) 381 WP_065214465.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  H7F24_RS14010 (H7F24_14010) - 2788169..2789017 (+) 849 WP_044335939.1 STAS domain-containing protein -
  H7F24_RS14015 (H7F24_14015) - 2789050..2789592 (-) 543 WP_185854432.1 IseA DL-endopeptidase inhibitor family protein -
  H7F24_RS14020 (H7F24_14020) - 2789887..2790330 (+) 444 WP_044335372.1 hypothetical protein -
  H7F24_RS14025 (H7F24_14025) lexA 2790375..2790995 (-) 621 WP_024427080.1 transcriptional repressor LexA -
  H7F24_RS14030 (H7F24_14030) yneA 2791154..2791465 (+) 312 WP_024425130.1 cell division suppressor protein YneA -
  H7F24_RS14035 (H7F24_14035) - 2791483..2792130 (+) 648 WP_025093247.1 recombinase family protein -
  H7F24_RS14040 (H7F24_14040) - 2792200..2792430 (+) 231 WP_024425132.1 DUF896 domain-containing protein -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13833.49 Da        Isoelectric Point: 7.9051

>NTDB_id=475464 H7F24_RS14005 WP_065214465.1 2787643..2788023(+) (nucA/comI) [Bacillus sp. PAMC22265]
MGTLFGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISEFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=475464 H7F24_RS14005 WP_065214465.1 2787643..2788023(+) (nucA/comI) [Bacillus sp. PAMC22265]
ATGGGCACGTTGTTTGGCGGATTTGGAGAGAAGCAGGCAGCAAAAGGGGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAAAGGTACCCTGAAACAGGCAGTCATATTCAAGAAGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCA
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTTCAATATGTCAGTCCATCTGATAATAGGGGGGCAGGCTCATG
GGTCGGGCATCAAATCAGCGAGTTTCCTGATGGGAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.983

94.444

0.548