Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JTR57_RS00500 Genome accession   NZ_CP070962
Coordinates   108842..109519 (-) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli strain K-12 J53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 103842..114519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JTR57_RS00485 chiA 105209..107902 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  JTR57_RS00490 bfd 108071..108265 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JTR57_RS00495 bfr 108337..108813 (+) 477 WP_000675504.1 bacterioferritin -
  JTR57_RS00500 pilD 108842..109519 (-) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  JTR57_RS00505 gspM 109519..109980 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  JTR57_RS00510 gspL 109977..111140 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  JTR57_RS00515 gspK 111155..112138 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  JTR57_RS00520 gspJ 112131..112718 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  JTR57_RS00525 gspI 112711..113088 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JTR57_RS00530 gspH 113085..113594 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  JTR57_RS00535 gspG 113602..114039 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=475173 JTR57_RS00500 WP_000178154.1 108842..109519(-) (pilD) [Escherichia coli strain K-12 J53]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=475173 JTR57_RS00500 WP_000178154.1 108842..109519(-) (pilD) [Escherichia coli strain K-12 J53]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378