Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JR883_RS01915 Genome accession   NZ_CP070930
Coordinates   429011..429688 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 30COLEC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 424011..434688
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JR883_RS01900 (JR883_01900) chiA 425378..428071 (+) 2694 WP_000773142.1 bifunctional chitinase/lysozyme -
  JR883_RS01905 (JR883_01905) bfd 428240..428434 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JR883_RS01910 (JR883_01910) bfr 428506..428982 (+) 477 WP_000675504.1 bacterioferritin -
  JR883_RS01915 (JR883_01915) pilD 429011..429688 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  JR883_RS01920 (JR883_01920) gspM 429688..430149 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  JR883_RS01925 (JR883_01925) gspL 430146..431309 (-) 1164 WP_001115235.1 type II secretion system protein GspL -
  JR883_RS01930 (JR883_01930) gspK 431324..432307 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  JR883_RS01935 (JR883_01935) gspJ 432300..432887 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  JR883_RS01940 (JR883_01940) gspI 432880..433257 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JR883_RS01945 (JR883_01945) gspH 433254..433763 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  JR883_RS01950 (JR883_01950) gspG 433771..434208 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=475098 JR883_RS01915 WP_000178161.1 429011..429688(-) (pilD) [Escherichia coli strain 30COLEC]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=475098 JR883_RS01915 WP_000178161.1 429011..429688(-) (pilD) [Escherichia coli strain 30COLEC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378