Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JR909_RS01300 Genome accession   NZ_CP070920
Coordinates   288400..289896 (+) Length   498 a.a.
NCBI ID   WP_001040690.1    Uniprot ID   -
Organism   Escherichia coli strain 40EC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 283400..294896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JR909_RS01270 (JR909_01270) tusA 283647..283892 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  JR909_RS01275 (JR909_01275) zntA 283994..286192 (-) 2199 WP_000106574.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  JR909_RS01280 (JR909_01280) yhhN 286266..286892 (-) 627 WP_000964718.1 lysoplasmalogenase -
  JR909_RS01285 (JR909_01285) yhhM 287033..287392 (+) 360 WP_000042873.1 DUF2500 domain-containing protein -
  JR909_RS01290 (JR909_01290) yhhL 287395..287664 (-) 270 WP_001295207.1 DUF1145 family protein -
  JR909_RS01295 (JR909_01295) rsmD 287654..288250 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  JR909_RS01300 (JR909_01300) pilA 288400..289896 (+) 1497 WP_001040690.1 signal recognition particle-docking protein FtsY Machinery gene
  JR909_RS01305 (JR909_01305) ftsE 289899..290567 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  JR909_RS01310 (JR909_01310) ftsX 290560..291618 (+) 1059 WP_097494619.1 permease-like cell division protein FtsX -
  JR909_RS01315 (JR909_01315) rpoH 291863..292717 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  JR909_RS01320 (JR909_01320) livJ 292988..294091 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  JR909_RS01325 (JR909_01325) panM 294290..294673 (-) 384 WP_000778804.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54671.44 Da        Isoelectric Point: 4.1468

>NTDB_id=475015 JR909_RS01300 WP_001040690.1 288400..289896(+) (pilA) [Escherichia coli strain 40EC]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=475015 JR909_RS01300 WP_001040690.1 288400..289896(+) (pilA) [Escherichia coli strain 40EC]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAAACGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACAAGCCGTCGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAACCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAAGCGGTGATAGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTTGAAGAAATTGCCCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
TAACAATATCCCGGTGATTGCCCAGCATACTGGTGCGGATTCCGCTTCGGTTATCTTCGACGCCATTCAGGCTGCGAAGG
CGCGTAATGTTGACGTACTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)

SRP54-type protein, GTPase domain (SRP54)

(294-494)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.571

77.309

0.375