Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JR895_RS02005 Genome accession   NZ_CP070902
Coordinates   435218..435895 (-) Length   225 a.a.
NCBI ID   WP_025670758.1    Uniprot ID   -
Organism   Escherichia coli strain 99COLEC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 430218..440895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JR895_RS01990 (JR895_01990) chiA 431585..434278 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  JR895_RS01995 (JR895_01995) bfd 434447..434641 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JR895_RS02000 (JR895_02000) bfr 434713..435189 (+) 477 WP_000675504.1 bacterioferritin -
  JR895_RS02005 (JR895_02005) pilD 435218..435895 (-) 678 WP_025670758.1 prepilin peptidase GspO Machinery gene
  JR895_RS02010 (JR895_02010) gspM 435895..436356 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  JR895_RS02015 (JR895_02015) gspL 436353..437516 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  JR895_RS02020 (JR895_02020) gspK 437531..438514 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  JR895_RS02025 (JR895_02025) gspJ 438507..439094 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  JR895_RS02030 (JR895_02030) gspI 439087..439464 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  JR895_RS02035 (JR895_02035) gspH 439461..439970 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  JR895_RS02040 (JR895_02040) gspG 439978..440415 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24888.75 Da        Isoelectric Point: 7.9563

>NTDB_id=474776 JR895_RS02005 WP_025670758.1 435218..435895(-) (pilD) [Escherichia coli strain 99COLEC]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLSDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=474776 JR895_RS02005 WP_025670758.1 435218..435895(-) (pilD) [Escherichia coli strain 99COLEC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGTCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378