Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   JOW61_RS13315 Genome accession   NZ_CP070844
Coordinates   2468655..2469065 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. natto strain BN0     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2463655..2474065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JOW61_RS13285 (JOW61_13175) - 2463743..2463883 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  JOW61_RS13290 (JOW61_13180) yqeF 2464189..2464920 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  JOW61_RS13295 (JOW61_13185) cwlH 2465172..2465924 (-) 753 WP_014480318.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  JOW61_RS13300 (JOW61_13190) yqeD 2466111..2466737 (+) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  JOW61_RS13305 (JOW61_13195) gnd 2466756..2467649 (-) 894 WP_014480320.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  JOW61_RS13310 (JOW61_13200) yqeB 2467900..2468622 (+) 723 WP_014480321.1 hypothetical protein -
  JOW61_RS13315 (JOW61_13205) nucA/comI 2468655..2469065 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  JOW61_RS13320 (JOW61_13210) sigK 2469261..2469989 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  JOW61_RS13325 (JOW61_13215) - 2469989..2470087 (+) 99 WP_031600702.1 hypothetical protein -
  JOW61_RS13330 - 2470084..2470296 (-) 213 Protein_2578 recombinase family protein -
  JOW61_RS13335 (JOW61_13225) fumC 2470515..2471903 (-) 1389 WP_014480325.1 class II fumarate hydratase -
  JOW61_RS13340 (JOW61_13230) - 2472070..2472960 (+) 891 WP_014480326.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=474715 JOW61_RS13315 WP_009967785.1 2468655..2469065(-) (nucA/comI) [Bacillus subtilis subsp. natto strain BN0]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=474715 JOW61_RS13315 WP_009967785.1 2468655..2469065(-) (nucA/comI) [Bacillus subtilis subsp. natto strain BN0]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529