Detailed information NTG0474707

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: I5155_RS27270
• Genome accession: NZ_CP060243
• Coordinates: 5755413..5756285 (+)
• Length: 290 a.a.
• NCBI ID: WP_015648060.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain A-I-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5750413..5761285

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5155_RS27255 (I5155_005603)	-	5751796..5752260 (-)	465	WP_023129290.1	pilin	-
I5155_RS27260 (I5155_005604)	pilB	5752491..5754191 (+)	1701	WP_030048379.1	type IV-A pilus assembly ATPase PilB	Machinery gene
I5155_RS27265 (I5155_005605)	pilC	5754195..5755412 (+)	1218	WP_015648061.1	type II secretion system F family protein	Machinery gene
I5155_RS27270 (I5155_005606)	pilD	5755413..5756285 (+)	873	WP_015648060.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
I5155_RS27275 (I5155_005607)	coaE	5756282..5756893 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
I5155_RS27280 (I5155_005608)	yacG	5756890..5757090 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
I5155_RS27285 (I5155_005609)	-	5757127..5757336 (-)	210	WP_003094660.1	hypothetical protein	-
I5155_RS27290 (I5155_005610)	-	5757442..5758131 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
I5155_RS27295 (I5155_005611)	-	5758128..5758598 (-)	471	WP_003103866.1	hypothetical protein	-
I5155_RS27300 (I5155_005612)	-	5758595..5759020 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
I5155_RS27305 (I5155_005613)	-	5759153..5759782 (+)	630	WP_015648058.1	DUF1780 domain-containing protein	-
I5155_RS27310 (I5155_005614)	-	5759779..5760228 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
I5155_RS27315 (I5155_005615)	-	5760254..5760424 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31889.86 Da
• Isoelectric Point: 7.3454

>NTDB_id=474707 I5155_RS27270 WP_015648060.1 5755413..5756285(+) (pilD) [Pseudomonas aeruginosa strain A-I-1]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGNCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGTVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=474707 I5155_RS27270 WP_015648060.1 5755413..5756285(+) (pilD) [Pseudomonas aeruginosa strain A-I-1]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAACTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCACGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55	96.552	0.531
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter nosocomialis M2	50.177	97.586	0.49
pilD	Acinetobacter baumannii D1279779	50.177	97.586	0.49
pilD	Neisseria gonorrhoeae MS11	48.201	95.862	0.462