Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   I5154_RS23620 Genome accession   NZ_CP060242
Coordinates   5024300..5025637 (+) Length   445 a.a.
NCBI ID   WP_003094694.1    Uniprot ID   A0A0H2ZG94
Organism   Pseudomonas aeruginosa strain B-I-1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5019300..5030637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5154_RS23595 (I5154_004850) pgeF 5019443..5020171 (-) 729 WP_003094684.1 peptidoglycan editing factor PgeF -
  I5154_RS23600 (I5154_004851) rluD 5020168..5021130 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  I5154_RS23605 (I5154_004852) - 5021276..5022301 (+) 1026 WP_003094687.1 outer membrane protein assembly factor BamD -
  I5154_RS23610 (I5154_004853) - 5022458..5022703 (+) 246 WP_014603563.1 PP0621 family protein -
  I5154_RS23615 (I5154_004854) pilS 5022693..5024285 (+) 1593 WP_003094692.1 two-component system sensor histidine kinase PilS Regulator
  I5154_RS23620 (I5154_004855) pilR 5024300..5025637 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  I5154_RS23625 (I5154_004856) thiO 5025683..5026777 (-) 1095 WP_003094695.1 glycine oxidase ThiO -
  I5154_RS23630 (I5154_004857) - 5026912..5027421 (+) 510 WP_015503652.1 GspH/FimT family pseudopilin -
  I5154_RS23635 (I5154_004858) - 5027527..5028033 (+) 507 WP_003094696.1 GspH/FimT family pseudopilin -
  I5154_RS23640 (I5154_004859) pilV 5028024..5028581 (+) 558 WP_003094697.1 type 4a pilus minor pilin PilV Machinery gene
  I5154_RS23645 (I5154_004860) pilW 5028578..5029402 (+) 825 WP_003094698.1 type 4a pilus minor pilin PilW -
  I5154_RS23650 (I5154_004861) pilX 5029399..5029986 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49737.06 Da        Isoelectric Point: 5.8654

>NTDB_id=474674 I5154_RS23620 WP_003094694.1 5024300..5025637(+) (pilR) [Pseudomonas aeruginosa strain B-I-1]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=474674 I5154_RS23620 WP_003094694.1 5024300..5025637(+) (pilR) [Pseudomonas aeruginosa strain B-I-1]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTAGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGCAGCGGCAAGGAACTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCACCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTATACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCACTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZG94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

100

100

1
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384