Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   I5102_RS25375 Genome accession   NZ_CP060240
Coordinates   5404836..5406428 (+) Length   530 a.a.
NCBI ID   WP_003123394.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain G-I-1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5399836..5411428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5102_RS25355 (I5102_005201) pgeF 5401586..5402314 (-) 729 WP_003102588.1 peptidoglycan editing factor PgeF -
  I5102_RS25360 (I5102_005202) rluD 5402311..5403273 (-) 963 WP_196331857.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  I5102_RS25365 (I5102_005203) - 5403419..5404444 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  I5102_RS25370 (I5102_005204) - 5404601..5404846 (+) 246 WP_003102591.1 PP0621 family protein -
  I5102_RS25375 (I5102_005205) pilS 5404836..5406428 (+) 1593 WP_003123394.1 two-component system sensor histidine kinase PilS Regulator
  I5102_RS25380 (I5102_005206) pilR 5406443..5407780 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  I5102_RS25385 (I5102_005207) thiO 5407826..5408920 (-) 1095 WP_003119424.1 glycine oxidase ThiO -
  I5102_RS25390 (I5102_005208) - 5409055..5409564 (+) 510 WP_003102595.1 GspH/FimT family pseudopilin -
  I5102_RS25395 (I5102_005209) - 5409670..5410176 (+) 507 WP_003102598.1 GspH/FimT family pseudopilin -
  I5102_RS25400 (I5102_005210) pilV 5410167..5410724 (+) 558 WP_003119423.1 type 4a pilus minor pilin PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58999.18 Da        Isoelectric Point: 6.6886

>NTDB_id=474608 I5102_RS25375 WP_003123394.1 5404836..5406428(+) (pilS) [Pseudomonas aeruginosa strain G-I-1]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SQLHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEIIDDGPGVPADKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=474608 I5102_RS25375 WP_003123394.1 5404836..5406428(+) (pilS) [Pseudomonas aeruginosa strain G-I-1]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCACGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTACCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCTTGGTCATCGCGGCGGCGGCCAGCCTCG
GTCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTTGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAC
AGCCAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACATCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAATCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAGCTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.811

100

0.998