Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   H7F27_RS15175 Genome accession   NZ_CP060193
Coordinates   2997450..3001154 (-) Length   1234 a.a.
NCBI ID   WP_185848105.1    Uniprot ID   A0A7G7UAK3
Organism   Bacillus sp. PAMC26543     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2992450..3006154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F27_RS15160 (H7F27_15160) - 2992495..2992797 (-) 303 WP_185848102.1 HNH endonuclease -
  H7F27_RS15165 (H7F27_15165) sbcC 2992811..2996203 (-) 3393 WP_185848103.1 exonuclease subunit SbcC -
  H7F27_RS15170 (H7F27_15170) sbcD 2996200..2997378 (-) 1179 WP_185848104.1 exonuclease subunit SbcD -
  H7F27_RS15175 (H7F27_15175) addA 2997450..3001154 (-) 3705 WP_185848105.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  H7F27_RS15180 (H7F27_15180) addB 3001141..3004641 (-) 3501 WP_185848106.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  H7F27_RS15185 (H7F27_15185) - 3004825..3005262 (+) 438 WP_044156482.1 ferritin family protein -
  H7F27_RS15190 (H7F27_15190) - 3005391..3005705 (+) 315 WP_185848107.1 four-helix bundle copper-binding protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141573.05 Da        Isoelectric Point: 5.1037

>NTDB_id=474528 H7F27_RS15175 WP_185848105.1 2997450..3001154(-) (addA) [Bacillus sp. PAMC26543]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPVDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEEEYAKGDKA
FFELVDRYTTDRHDLDLQYLVKQVYEYSRSHPDPETWLKSFVHLYDVTEKSAIEELPFYPYVKEDIAMVLDGAKEKLQRA
LELTKAPGGPAPRAENFLDDLAQIDDLIQHQDDFNELYQRVPAVSFKRAKAVKGDEFDPVLLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHVKSLAEMEPVIETLVQLVISYGKRFEAAKQEKSLIDFSDLEHYCLSILTAVNEKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDDQAELKLGAAYPENNETETELLLIDKSEEADAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVSVVLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKDHQKQLAKWQASASQADWLLPEFDRYQAKTYLDFIGPALVRHRDLESLAEGGAAAVSDISGHPARFAIQMIHSYD
LLDDELEERLEEKSERLEAIRRGEPVPGPFAFEEKARERLAWKYSYHEVTQIRTKQSVSEIKRRREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAERTVQHLYEKELLTEEQQSAINIEEIVQFFHTEIGE
KLIGAKWKNREVPFSLALPAKEIYPDAKEADEPLLVQGIIDCLYETEDGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTRAVEQIAQTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=474528 H7F27_RS15175 WP_185848105.1 2997450..3001154(-) (addA) [Bacillus sp. PAMC26543]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAGTGGAATGCCATCGTCTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCGGCAGGCTCCGGTAAAACCGCTGTTCTTGTCGAAAGAATGATCCGGAAAATCACGGCGGAAGAAAATC
CGGTTGATGTAGACCGGCTTCTCGTTGTCACCTTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCA
CTGGAAAAAGAGCTGGTAAAACGCCCAGGATCACTGCATATCCGACGCCAGCTGTCCCTATTAAACCGGGCCAGCATTTC
AACGCTTCATTCGTTTTGCCTGCAGGTGCTGAAAAAATATTACTACCTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTTCTCGGGGACGAGGTGCTGGATGAGCTGTTTGAGGAGGAATATGCAAAGGGTGATAAGGCG
TTCTTTGAGCTTGTCGACCGCTACACGACTGACCGGCATGACTTAGATCTGCAATATCTCGTGAAACAGGTGTACGAGTA
TTCCCGTTCCCACCCTGATCCGGAGACATGGCTGAAAAGTTTTGTTCATTTGTATGATGTAACAGAAAAGAGCGCGATCG
AGGAACTGCCGTTTTATCCGTATGTCAAAGAAGATATTGCGATGGTGCTGGACGGAGCAAAGGAAAAGCTCCAGCGTGCC
CTTGAGCTGACAAAAGCGCCGGGAGGACCGGCGCCGCGCGCTGAAAATTTTCTCGATGATCTCGCGCAGATTGATGACCT
GATTCAGCATCAGGATGACTTCAATGAACTTTATCAGCGCGTGCCCGCTGTCTCCTTTAAGCGTGCAAAAGCGGTGAAGG
GGGACGAGTTTGATCCCGTCTTGCTTGATGAAGCGACAGACTTACGAAATGGCGCCAAAAAACTGCTTGAAAAACTCAAA
ACCGATTACTTCACCAGAAGCCCGGAGCAGCATGTAAAAAGTTTGGCTGAGATGGAGCCTGTCATTGAAACACTCGTCCA
GCTTGTGATCAGCTACGGGAAACGGTTTGAAGCCGCGAAGCAGGAGAAATCGCTCATTGATTTTTCTGATTTGGAGCATT
ATTGCCTGTCCATCTTGACCGCTGTGAACGAAAAAGGCGAACGTGAGCCGAGTGAGGCGGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTTGTCGATGAATATCAGGACACCAACCTGGTGCAGGAATCCATTCTTCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGAAACCTGTTTATGGTAGGAGATGTCAAACAGTCAATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TTCTCTCCAAGTACAAACGGTTTACAGAGAGCGGAGAAGGTACGGGCCGGAAAATCGATTTGAACAAAAACTTTCGAAGC
AGGGCTGATATTTTAGACAGCACAAACTTTTTGTTCAAACAGCTGATGGGCGGCAAAATCGGCGAGGTCGATTATGATGA
TCAGGCTGAGCTGAAACTCGGAGCGGCGTATCCGGAAAACAACGAAACGGAAACAGAGCTGCTGCTGATTGACAAGTCAG
AGGAGGCGGACGCAGGCGAGGAAGCGGAAGAGCTGGAAACGGTGCAGTTCGAAGCAAAAGCGATCGCCAAGGAAATACGC
AAGCTGATCTCATCGCCATTTAAGGTATATGACGGGAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTGCGATCCATGCCTTGGGCGCCGCAAATCATGGAAGAACTGCGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CATCAGGATACTTTGAGGCGGTGGAAGTGTCTGTGGTGCTTTCTGTCCTGAAGGTGATTGATAACCCGTATCAGGATATT
CCGCTTGCTTCCGTTCTGCGTTCACCGATTGTCGGTGCTGACGAGAACGAACTGTCTTTGATCCGGCTTGAAAACAAAAA
GGCGCCGTACTATGAAGCAATGAAAGACTATCTGGCAGCCGGCGACCGAAACGATGAGCTCTACCAAAAGCTGCAAACGT
TTTACGGACATTTGCAAAAGTGGCGCGCATTTTCGAAAAACCATTCGGTGTCCGAGCTGATCTGGGAAGTGTACCGCGAC
ACAAAATATATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCTAACTTGCGTGTCCTTTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGCTTCTTGAGGTTTATCGAACGCATGCAGGAACGCGGCGATG
ACCTAGGAACGGCGAGAGCGTTAAGCGAACAGGAGGATGTTGTCCGTTTAATGACGATTCACAGCAGCAAAGGACTCGAA
TTTCCAGTCGTCTTCACAGCGGGACTCGGCCGGAATTTCAACATGATGGATCTCAACAAATCCTACCTGCTGGACAAGGA
GCTTGGGTTTGGCACGAAATTTATCCATCCTGAATGGCGCATCAGCTATCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGGGAACTGCTGTCTGAGGAATTGCGTGTCCTCTATGTTGCTTTAACGAGGGCAAAGGAAAAGCTGTTTCTT
GTAGGCTCATGCAAGGATCATCAGAAACAGCTGGCAAAATGGCAGGCATCCGCGTCCCAAGCTGACTGGCTTCTGCCGGA
ATTTGACCGCTATCAGGCGAAAACGTATTTGGATTTCATCGGACCTGCTCTTGTCAGACACAGAGACTTGGAGTCTTTGG
CTGAGGGCGGCGCGGCAGCGGTTTCTGATATATCAGGCCATCCGGCCCGCTTTGCTATTCAAATGATCCATTCATATGAT
CTGCTTGATGATGAGCTGGAAGAGAGACTGGAAGAAAAAAGCGAACGTCTTGAAGCGATCCGCCGTGGTGAACCGGTTCC
CGGCCCGTTTGCATTTGAGGAAAAAGCTCGCGAGCGCCTGGCCTGGAAGTATTCGTATCACGAGGTGACACAAATTCGCA
CGAAGCAGTCCGTTTCTGAGATTAAGAGAAGAAGAGAGTACGAAGACGAATACAGCGGACGGGCGCCCGTTAAACCGGCT
GATGGAAGCATTCTGTACAGACGCCCGGCTTTCATGATGAAAAAAGGCCTGACAGCAGCAGAAAAAGGAACAGCTATGCA
TACGGTCATGCAGCATATTCCGCTGACACATGTGCCGACGATAGAAGAAGCGGAACGGACAGTGCAGCATCTTTATGAAA
AAGAGCTGCTCACCGAAGAACAGCAATCCGCTATCAATATTGAGGAAATCGTTCAATTTTTCCATACGGAAATTGGCGAA
AAACTGATCGGCGCTAAATGGAAGAACCGCGAAGTTCCATTCAGTTTGGCGCTTCCGGCAAAAGAGATTTATCCTGATGC
GAAAGAGGCGGATGAGCCGCTATTGGTGCAGGGGATCATCGATTGTCTCTACGAAACAGAGGATGGCTTATATTTGCTGG
ACTATAAAACAGACCGAATTGAGGGTAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCCTGAAAAAGAGATATGAA
ACGCAAATTCAGCTGTACACAAGGGCCGTTGAACAAATTGCTCAAACGAAGGTAAAAGGGTGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACTTTATAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G7UAK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

93.355

100

0.934