Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H7F27_RS08130 Genome accession   NZ_CP060193
Coordinates   1612766..1613191 (+) Length   141 a.a.
NCBI ID   WP_101861068.1    Uniprot ID   -
Organism   Bacillus sp. PAMC26543     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1607766..1618191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F27_RS08100 (H7F27_08100) - 1608601..1608773 (+) 173 Protein_1586 recombinase family protein -
  H7F27_RS08105 (H7F27_08105) - 1608916..1610133 (-) 1218 WP_106020130.1 CynX/NimT family MFS transporter -
  H7F27_RS08110 (H7F27_08110) - 1610277..1610834 (+) 558 WP_101861070.1 XRE family transcriptional regulator -
  H7F27_RS21125 - 1611459..1611618 (+) 160 Protein_1589 recombinase family protein -
  H7F27_RS08125 (H7F27_08125) sigK 1611861..1612589 (-) 729 WP_174754429.1 RNA polymerase sporulation sigma factor SigK -
  H7F27_RS08130 (H7F27_08130) nucA/comI 1612766..1613191 (+) 426 WP_101861068.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  H7F27_RS08135 (H7F27_08135) - 1613225..1613953 (-) 729 WP_185848805.1 DUF308 domain-containing protein -
  H7F27_RS08140 (H7F27_08140) gnd 1614274..1615167 (+) 894 WP_101861066.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  H7F27_RS08145 (H7F27_08145) - 1615189..1615815 (-) 627 WP_024122122.1 TVP38/TMEM64 family protein -
  H7F27_RS08150 (H7F27_08150) - 1616010..1616762 (+) 753 WP_185848806.1 N-acetylmuramoyl-L-alanine amidase -
  H7F27_RS08155 (H7F27_08155) - 1617009..1617740 (+) 732 WP_024122120.1 SGNH/GDSL hydrolase family protein -
  H7F27_RS20840 - 1617818..1617952 (+) 135 WP_010335019.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15470.65 Da        Isoelectric Point: 5.7516

>NTDB_id=474495 H7F27_RS08130 WP_101861068.1 1612766..1613191(+) (nucA/comI) [Bacillus sp. PAMC26543]
MKRWMAMLLLAAAVFLCLLGKDMTAPQQAKGAALYDKILYFPLSRYPETGDHIRDAIAEGHSDICTIDRGGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=474495 H7F27_RS08130 WP_101861068.1 1612766..1613191(+) (nucA/comI) [Bacillus sp. PAMC26543]
ATGAAAAGATGGATGGCAATGCTTCTTCTGGCGGCAGCAGTTTTTCTTTGTTTGTTGGGAAAGGATATGACGGCTCCGCA
ACAGGCCAAGGGGGCTGCTTTATATGACAAAATTTTATACTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCAATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGGTGGAGCGGACAAAAGGCGGGAGGAATCATTA
AAAGGAATTCCAACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGCGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCAGATGGCA
CCAAAGTGTTGTTTATTGTTCAGTGA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

60.484

87.943

0.532