Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H7F26_RS16475 Genome accession   NZ_CP060192
Coordinates   3199752..3200180 (+) Length   142 a.a.
NCBI ID   WP_003182220.1    Uniprot ID   A0A1Y0YFI7
Organism   Bacillus sp. PAMC28748     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3194752..3205180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F26_RS16445 (H7F26_16445) - 3194935..3195543 (+) 609 WP_009328237.1 flavin reductase family protein -
  H7F26_RS16450 (H7F26_16450) wrbA 3195744..3196355 (+) 612 WP_003182210.1 NAD(P)H:quinone oxidoreductase -
  H7F26_RS16455 (H7F26_16455) - 3196676..3196816 (+) 141 WP_003182211.1 hypothetical protein -
  H7F26_RS16460 (H7F26_16460) - 3196809..3197642 (+) 834 WP_003182213.1 prohibitin family protein -
  H7F26_RS16465 (H7F26_16465) - 3197733..3198494 (+) 762 WP_003182215.1 hypothetical protein -
  H7F26_RS16470 (H7F26_16470) - 3198554..3199393 (+) 840 WP_003182218.1 thymidylate synthase -
  H7F26_RS16475 (H7F26_16475) nucA/comI 3199752..3200180 (+) 429 WP_003182220.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  H7F26_RS16480 (H7F26_16480) lexA 3200376..3200999 (-) 624 WP_003182222.1 transcriptional repressor LexA -
  H7F26_RS16485 (H7F26_16485) yneA 3201152..3201463 (+) 312 WP_003182225.1 cell division suppressor protein YneA -
  H7F26_RS16490 (H7F26_16490) - 3201482..3202129 (+) 648 WP_003182227.1 recombinase family protein -
  H7F26_RS16495 (H7F26_16495) - 3202195..3202428 (+) 234 WP_003182231.1 DUF896 domain-containing protein -
  H7F26_RS16500 (H7F26_16500) tkt 3202595..3204598 (+) 2004 WP_009328229.1 transketolase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15332.29 Da        Isoelectric Point: 7.8987

>NTDB_id=474449 H7F26_RS16475 WP_003182220.1 3199752..3200180(+) (nucA/comI) [Bacillus sp. PAMC28748]
MIKKWAVHLLFSALVLLGLSGGAAYSPQHAEGAARYDDILYFPASRYPETGAHISDAIKAGHSDVCTIERSGADKRRQES
LKGIPTKPGFDRDEWPMAMCEEGGKGASVRYVSSSDNRGAGSWVGNRLSGFADGTRILFIVQ

Nucleotide


Download         Length: 429 bp        

>NTDB_id=474449 H7F26_RS16475 WP_003182220.1 3199752..3200180(+) (nucA/comI) [Bacillus sp. PAMC28748]
ATGATCAAAAAATGGGCGGTTCATCTGCTGTTTTCCGCATTGGTACTGCTTGGGCTTTCGGGAGGCGCCGCATATTCTCC
TCAGCATGCCGAAGGTGCTGCAAGGTATGACGACATATTGTATTTTCCGGCATCACGCTATCCCGAAACCGGCGCTCATA
TCAGCGACGCAATCAAAGCGGGGCATTCAGATGTCTGCACGATTGAAAGATCGGGAGCGGATAAGCGCCGCCAGGAATCA
CTGAAGGGGATTCCGACTAAGCCGGGCTTTGACCGTGACGAATGGCCGATGGCCATGTGTGAAGAAGGGGGCAAAGGAGC
GTCTGTCAGATATGTCAGCTCATCGGATAACCGCGGAGCCGGCTCCTGGGTCGGGAACAGGCTGAGCGGTTTCGCCGACG
GGACGAGAATTTTGTTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0YFI7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.73

88.732

0.521