Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H7F25_RS00250 Genome accession   NZ_CP060191
Coordinates   51612..52040 (-) Length   142 a.a.
NCBI ID   WP_024424305.1    Uniprot ID   A0A5C0WGQ8
Organism   Bacillus sp. PAMC28571     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 46612..57040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7F25_RS00220 (H7F25_00220) - 46775..47104 (+) 330 WP_185854516.1 YckD family protein -
  H7F25_RS00225 (H7F25_00225) - 47148..47912 (-) 765 WP_034624002.1 MBL fold metallo-hydrolase -
  H7F25_RS00230 (H7F25_00230) - 48028..48444 (+) 417 WP_025092811.1 Lrp/AsnC family transcriptional regulator -
  H7F25_RS00235 (H7F25_00235) - 48473..49279 (+) 807 WP_185854078.1 aminoglycoside N(3)-acetyltransferase -
  H7F25_RS00240 (H7F25_00240) - 49281..49667 (-) 387 WP_041106618.1 competence protein ComJ -
  H7F25_RS19485 - 49787..50734 (-) 948 WP_370880873.1 methyl-accepting chemotaxis protein -
  H7F25_RS19490 - 50774..51541 (-) 768 Protein_49 cache domain-containing protein -
  H7F25_RS00250 (H7F25_00250) nucA/comI 51612..52040 (-) 429 WP_024424305.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  H7F25_RS00255 (H7F25_00255) - 52408..54477 (+) 2070 WP_185854080.1 hydantoinase/oxoprolinase family protein -
  H7F25_RS00260 (H7F25_00260) - 54467..56455 (+) 1989 WP_185854081.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15971.96 Da        Isoelectric Point: 6.2215

>NTDB_id=474333 H7F25_RS00250 WP_024424305.1 51612..52040(-) (nucA/comI) [Bacillus sp. PAMC28571]
MKLLKMILLLLLIVIGVATGYIQLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDRS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVRPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=474333 H7F25_RS00250 WP_024424305.1 51612..52040(-) (nucA/comI) [Bacillus sp. PAMC28571]
ATGAAACTTCTTAAAATGATTCTCTTGCTTTTATTGATTGTTATTGGTGTAGCAACAGGCTATATCCAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACGATTCATTTTCCATCAGACCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCATCCATTTGTACAATTGACCGTAAACATAGTGATGAGCAGAGGGATCGATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGTGACGAATGGCCAATGGCAATGTGCAAAGAAGGAGGCACAGGAGC
TTCTGTCAAATATGTACGCCCTTCTGATAATAGAGGCGCAGGCTCGTGGGTGAGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATTCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C0WGQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.667

80.282

0.535