Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   LBHH_RS09455 Genome accession   NC_017467
Coordinates   1872943..1874739 (+) Length   598 a.a.
NCBI ID   WP_014564220.1    Uniprot ID   -
Organism   Lactobacillus helveticus H10     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1854215..1887661 1872943..1874739 within 0


Gene organization within MGE regions


Location: 1854215..1887661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LBHH_RS09345 (LBHH_1756) - 1854215..1855018 (-) 804 WP_148224881.1 hypothetical protein -
  LBHH_RS09350 (LBHH_1757) - 1855156..1856088 (-) 933 WP_014564205.1 C40 family peptidase -
  LBHH_RS09355 (LBHH_1758) - 1856241..1857017 (-) 777 WP_041809730.1 C40 family peptidase -
  LBHH_RS09360 - 1857190..1857597 (-) 408 WP_238525420.1 DUF234 domain-containing protein -
  LBHH_RS09365 (LBHH_1759) - 1857615..1857863 (-) 249 WP_238525421.1 ATPase -
  LBHH_RS09370 (LBHH_1760) - 1857980..1858531 (-) 552 WP_014564208.1 C40 family peptidase -
  LBHH_RS09375 (LBHH_1761) - 1858721..1859266 (-) 546 WP_041809733.1 guanylate kinase -
  LBHH_RS09380 (LBHH_1762) - 1859361..1859660 (+) 300 WP_003627299.1 DUF2187 domain-containing protein -
  LBHH_RS09385 (LBHH_1763) - 1859753..1861258 (+) 1506 WP_014564210.1 O-antigen ligase family protein -
  LBHH_RS09390 (LBHH_1764) - 1861357..1862586 (-) 1230 WP_080553183.1 RNA-guided endonuclease TnpB family protein -
  LBHH_RS09395 (LBHH_1765) - 1862725..1863348 (-) 624 WP_023192898.1 LVIS_2131 family protein -
  LBHH_RS09400 (LBHH_1766) nrdI 1863474..1863920 (+) 447 WP_003627304.1 class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI -
  LBHH_RS13065 - 1863923..1864094 (+) 172 Protein_1874 ribonucleotide-diphosphate reductase subunit beta -
  LBHH_RS09405 - 1864065..1864247 (-) 183 Protein_1875 hypothetical protein -
  LBHH_RS09410 (LBHH_1767) - 1864516..1865052 (-) 537 WP_003627306.1 hypothetical protein -
  LBHH_RS09415 (LBHH_1768) - 1865253..1866533 (-) 1281 WP_014564213.1 RNA-guided endonuclease TnpB family protein -
  LBHH_RS09420 (LBHH_1769) - 1866896..1867381 (-) 486 WP_014564214.1 threonine/serine exporter family protein -
  LBHH_RS09425 (LBHH_1770) - 1867384..1868154 (-) 771 WP_014564215.1 threonine/serine exporter family protein -
  LBHH_RS09430 (LBHH_1771) - 1868165..1869706 (-) 1542 WP_014564216.1 ABC-F family ATP-binding cassette domain-containing protein -
  LBHH_RS09435 (LBHH_1772) - 1869715..1870092 (-) 378 WP_003627310.1 hypothetical protein -
  LBHH_RS09440 (LBHH_1773) - 1870226..1870783 (+) 558 WP_003627311.1 GNAT family N-acetyltransferase -
  LBHH_RS09445 - 1870780..1871471 (-) 692 Protein_1883 DUF554 domain-containing protein -
  LBHH_RS09450 (LBHH_1775) - 1871540..1872781 (-) 1242 WP_014564219.1 LCP family protein -
  LBHH_RS09455 (LBHH_1776) pepF 1872943..1874739 (+) 1797 WP_014564220.1 oligoendopeptidase F Regulator
  LBHH_RS12890 (LBHH_1777) - 1874875..1875006 (+) 132 WP_003627320.1 hypothetical protein -
  LBHH_RS12730 - 1875175..1875477 (+) 303 Protein_1887 Fic family protein -
  LBHH_RS09465 (LBHH_1778) - 1875616..1876840 (+) 1225 Protein_1888 RNA-guided endonuclease TnpB family protein -
  LBHH_RS09470 (LBHH_1779) - 1877058..1877330 (-) 273 WP_003631064.1 bacteriocin immunity protein -
  LBHH_RS09475 - 1877323..1878635 (-) 1313 Protein_1890 L-lactate permease -
  LBHH_RS09480 (LBHH_1782) - 1878872..1879246 (+) 375 Protein_1891 transposase -
  LBHH_RS09485 (LBHH_1783) - 1879473..1880855 (-) 1383 WP_014564224.1 L-cystine transporter -
  LBHH_RS09490 (LBHH_1784) - 1881291..1882085 (-) 795 WP_014564225.1 metal ABC transporter permease -
  LBHH_RS09495 (LBHH_1785) - 1882085..1882732 (-) 648 WP_014564226.1 metal ABC transporter ATP-binding protein -
  LBHH_RS09500 (LBHH_1786) - 1882764..1883681 (-) 918 WP_014564227.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LBHH_RS09505 (LBHH_1787) - 1883944..1884216 (-) 273 WP_014564228.1 ArsR/SmtB family transcription factor -
  LBHH_RS09510 (LBHH_1788) - 1884446..1885723 (+) 1278 WP_041809741.1 ISL3 family transposase -
  LBHH_RS09515 - 1885791..1886007 (-) 217 Protein_1898 hypothetical protein -
  LBHH_RS12895 - 1886136..1886267 (-) 132 WP_274377419.1 hypothetical protein -
  LBHH_RS09520 (LBHH_1789) - 1886270..1887661 (-) 1392 WP_014564230.1 ATP-binding protein -

Sequence

Protein


Download         Length: 598 a.a.        Molecular weight: 68110.08 Da        Isoelectric Point: 4.7092

>NTDB_id=47399 LBHH_RS09455 WP_014564220.1 1872943..1874739(+) (pepF) [Lactobacillus helveticus H10]
MAILTRSEVPEDLKWDLTRIFKTDQDWENAFDKAKDDVAKLSELKGSLAKSGKDLYEGLTKILAVKRDVENIYVYATMSS
DVDTSNSHYLGYVSRVQSLSNQFEAATSFINPEILSIPAEKFEQFKKDEPRLADYAHYLEMITNKRPHTLPAEEEKIIAD
AGDAMSVSENTFNVLTNSDMEYGYVQDEDGNMEQLSDGLYSLLIQSQNRDVRKGAFNTLYASYGQFQNSLASTLSGVVKK
HNYNARMHKYDSAREAALADNGVPVEVYDTLIKEVDSHLDLLHRYVALRKKILGLKDLQMWDMYVPLTGKPALSYNFEEA
KKVAKEALKPLGEDYLKHVDYIFNNRVIDPVESKGKVTGAYSGGAYDTDPYELLNWEDNIDSLYTLVHETGHSVHSWYTR
HSQPYIYGDYPIFVAEIASTTNENILTEYFLDHITDPKTRAFILNYYLDSFKGTLFRQTQFAVFEQFIHEADAKGEPLTA
DILDDVYGQINQHYYGDSVEPGGDIALEWSRIPHFYYNFYVYQYATGFAAATALANKVVHGSQADRDAYLGYLKSGSSDY
PTEIMKRAGVDMTKPDYLEDAFKTFEKRLNEFESLIGK

Nucleotide


Download         Length: 1797 bp        

>NTDB_id=47399 LBHH_RS09455 WP_014564220.1 1872943..1874739(+) (pepF) [Lactobacillus helveticus H10]
ATGGCGATTCTAACAAGAAGCGAAGTCCCAGAAGATTTGAAGTGGGATTTAACCCGCATCTTTAAAACAGACCAAGACTG
GGAAAATGCCTTTGACAAGGCAAAAGATGACGTGGCTAAGCTAAGTGAATTAAAGGGAAGCTTGGCTAAATCAGGCAAAG
ATTTGTATGAAGGCTTGACCAAGATTTTGGCAGTTAAACGTGATGTAGAAAATATTTACGTTTATGCCACTATGTCTAGC
GATGTTGATACTTCTAACTCACATTATTTGGGCTACGTTAGCCGCGTGCAAAGCTTGTCCAATCAATTTGAAGCAGCAAC
CAGTTTTATTAATCCTGAAATTTTGAGTATCCCTGCCGAAAAGTTTGAACAATTCAAAAAAGACGAGCCAAGATTAGCTG
ATTACGCCCACTATTTGGAAATGATCACTAACAAGCGTCCTCATACTTTGCCAGCAGAAGAAGAAAAAATTATCGCTGAC
GCAGGGGATGCCATGAGCGTGTCAGAGAATACCTTTAACGTTTTAACCAACTCTGACATGGAATATGGTTATGTGCAAGA
TGAAGACGGCAACATGGAGCAATTATCCGATGGCTTGTATTCATTATTGATTCAGTCCCAAAATCGTGACGTGCGTAAAG
GTGCTTTTAATACTCTCTATGCCAGCTATGGTCAATTCCAAAACTCGCTTGCCTCTACTCTCTCCGGCGTTGTGAAAAAA
CATAACTACAACGCACGCATGCACAAGTATGATTCAGCTCGTGAAGCCGCATTAGCTGATAACGGCGTACCTGTTGAAGT
TTACGACACATTAATTAAAGAAGTTGATTCACACCTTGACTTGCTTCACCGTTATGTCGCATTGCGCAAGAAAATTTTAG
GTCTTAAAGACTTACAAATGTGGGACATGTACGTGCCGCTAACTGGTAAGCCTGCTTTGTCTTACAACTTTGAAGAGGCT
AAAAAGGTAGCTAAAGAAGCCTTGAAGCCACTTGGCGAAGACTACTTAAAGCATGTTGATTATATTTTTAACAACCGTGT
GATTGACCCTGTTGAATCTAAGGGCAAGGTTACTGGTGCTTACTCTGGTGGTGCTTACGATACCGATCCATATGAACTTT
TGAACTGGGAAGACAATATCGATTCACTCTATACTTTAGTTCATGAAACTGGACACTCAGTTCACTCTTGGTACACCCGC
CACAGTCAGCCTTATATCTACGGTGATTACCCAATCTTCGTGGCTGAAATTGCTTCAACCACTAATGAAAATATTTTGAC
TGAATATTTCTTGGACCATATTACTGATCCTAAGACGCGCGCATTCATCTTGAACTACTACCTTGATTCATTCAAGGGTA
CATTGTTCCGCCAAACTCAATTTGCGGTATTTGAACAATTTATCCACGAAGCAGATGCTAAGGGCGAACCATTGACTGCC
GATATTTTGGATGATGTTTATGGTCAAATTAACCAGCATTACTACGGCGACAGTGTTGAACCTGGTGGCGATATTGCGCT
TGAATGGTCACGAATTCCGCACTTCTACTACAACTTCTACGTTTATCAATATGCGACTGGATTTGCGGCTGCAACGGCTT
TGGCTAACAAGGTTGTGCATGGTAGTCAGGCTGATAGGGATGCATACCTGGGCTACCTTAAGTCAGGTTCTAGTGACTAT
CCTACTGAGATCATGAAGCGTGCCGGCGTTGACATGACTAAGCCCGATTATTTGGAAGATGCTTTCAAGACTTTTGAAAA
GAGATTGAACGAATTCGAGAGTTTGATTGGTAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(203-583)

Oligopeptidase F (Peptidase_M3_N)

(113-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.515

99.331

0.512

Multiple sequence alignment