Detailed information NTG0473788

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: H5405_RS07115
• Genome accession: NZ_CP060085
• Coordinates: 1392789..1393403 (+)
• Length: 204 a.a.
• NCBI ID: WP_007612692.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain HAB-2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1387789..1398403

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H5405_RS07080 (H5405_07080)	aroE	1388476..1389318 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
H5405_RS07085 (H5405_07085)	yhbY	1389312..1389602 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
H5405_RS07090 (H5405_07090)	-	1389613..1390182 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
H5405_RS07095 (H5405_07095)	yqeK	1390172..1390732 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
H5405_RS07100 (H5405_07100)	rsfS	1390750..1391106 (+)	357	WP_007408260.1	ribosome silencing factor	-
H5405_RS07105 (H5405_07105)	-	1391103..1391840 (+)	738	WP_007612694.1	class I SAM-dependent methyltransferase	-
H5405_RS07110 (H5405_07110)	comER	1391909..1392730 (-)	822	WP_007612693.1	late competence protein ComER	-
H5405_RS07115 (H5405_07115)	comEA	1392789..1393403 (+)	615	WP_007612692.1	helix-hairpin-helix domain-containing protein	Machinery gene
H5405_RS07120 (H5405_07120)	-	1393470..1394039 (+)	570	WP_003152868.1	ComE operon protein 2	-
H5405_RS07125 (H5405_07125)	comEC	1394040..1396391 (+)	2352	WP_007612689.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
H5405_RS07130 (H5405_07130)	-	1396410..1396544 (-)	135	WP_029326064.1	YqzM family protein	-
H5405_RS07135 (H5405_07135)	-	1396585..1396736 (+)	152	Protein_1407	hypothetical protein	-
H5405_RS07140 (H5405_07140)	holA	1396776..1397817 (+)	1042	Protein_1408	DNA polymerase III subunit delta	-
H5405_RS07145 (H5405_07145)	rpsT	1397834..1398100 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22265.52 Da
• Isoelectric Point: 7.7754

>NTDB_id=473788 H5405_RS07115 WP_007612692.1 1392789..1393403(+) (comEA) [Bacillus velezensis strain HAB-2]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELKVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=473788 H5405_RS07115 WP_007612692.1 1392789..1393403(+) (comEA) [Bacillus velezensis strain HAB-2]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAAAGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363