Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G7042_RS20150 Genome accession   NZ_CP060030
Coordinates   4259735..4261174 (+) Length   479 a.a.
NCBI ID   WP_005314410.1    Uniprot ID   A4SHX7
Organism   Aeromonas salmonicida subsp. masoucida strain BR19001YR     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4254735..4266174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7042_RS20120 (G7042_20105) mlaF 4255312..4256112 (-) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -
  G7042_RS20125 (G7042_20110) - 4256320..4257315 (+) 996 WP_011898279.1 KpsF/GutQ family sugar-phosphate isomerase -
  G7042_RS20130 (G7042_20115) kdsC 4257315..4257869 (+) 555 WP_042468044.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  G7042_RS20135 (G7042_20120) lptC 4257866..4258426 (+) 561 WP_042468042.1 LPS export ABC transporter periplasmic protein LptC -
  G7042_RS20140 (G7042_20125) lptA 4258413..4258940 (+) 528 WP_005314414.1 lipopolysaccharide transport periplasmic protein LptA -
  G7042_RS20145 (G7042_20130) lptB 4258944..4259669 (+) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  G7042_RS20150 (G7042_20135) rpoN/rpoN1 4259735..4261174 (+) 1440 WP_005314410.1 RNA polymerase factor sigma-54 Machinery gene
  G7042_RS20155 (G7042_20140) hpf 4261196..4261483 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  G7042_RS20160 (G7042_20145) ptsN 4261486..4261932 (+) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  G7042_RS20165 (G7042_20150) rapZ 4261975..4262841 (+) 867 WP_005314405.1 RNase adapter RapZ -
  G7042_RS20170 (G7042_20155) npr 4262862..4263134 (+) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  G7042_RS20175 (G7042_20160) - 4263153..4265057 (-) 1905 WP_042468040.1 transcription antiterminator -
  G7042_RS20180 (G7042_20165) - 4265161..4265901 (-) 741 WP_005314402.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53962.92 Da        Isoelectric Point: 4.3718

>NTDB_id=473494 G7042_RS20150 WP_005314410.1 4259735..4261174(+) (rpoN/rpoN1) [Aeromonas salmonicida subsp. masoucida strain BR19001YR]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGRECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=473494 G7042_RS20150 WP_005314410.1 4259735..4261174(+) (rpoN/rpoN1) [Aeromonas salmonicida subsp. masoucida strain BR19001YR]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGACG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGACAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAACTGCCGGTAGATACCACCTGGGAAGATGTCTATTCAGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAAGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCCGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCAGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTAATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTCAAGAAGGGCAACAAATGGGTCGTTGAGCTCAACCCTG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACCAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAAGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A4SHX7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.58

100

0.484