Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6K13_RS20145 Genome accession   NZ_CP070162
Coordinates   4117820..4118497 (+) Length   225 a.a.
NCBI ID   WP_134316771.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1283     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4112820..4123497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K13_RS20110 (I6K13_20105) gspG 4113300..4113737 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  I6K13_RS20115 (I6K13_20110) gspH 4113745..4114254 (+) 510 WP_001076035.1 type II secretion system minor pseudopilin GspH -
  I6K13_RS20120 (I6K13_20115) gspI 4114251..4114628 (+) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  I6K13_RS20125 (I6K13_20120) gspJ 4114621..4115208 (+) 588 WP_021524559.1 type II secretion system minor pseudopilin GspJ -
  I6K13_RS20130 (I6K13_20125) gspK 4115201..4116184 (+) 984 WP_001058507.1 type II secretion system minor pseudopilin GspK -
  I6K13_RS20135 (I6K13_20130) gspL 4116199..4117362 (+) 1164 WP_001335091.1 type II secretion system protein GspL -
  I6K13_RS20140 (I6K13_20135) gspM 4117359..4117820 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  I6K13_RS20145 (I6K13_20140) pilD 4117820..4118497 (+) 678 WP_134316771.1 prepilin peptidase GspO Machinery gene
  I6K13_RS20150 (I6K13_20145) bfr 4118526..4119002 (-) 477 WP_000675504.1 bacterioferritin -
  I6K13_RS20155 (I6K13_20150) bfd 4119074..4119268 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6K13_RS20160 (I6K13_20155) chiA 4119437..4122139 (-) 2703 WP_021538187.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24828.66 Da        Isoelectric Point: 7.6364

>NTDB_id=473406 I6K13_RS20145 WP_134316771.1 4117820..4118497(+) (pilD) [Escherichia coli strain FDAARGOS_1283]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLGHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=473406 I6K13_RS20145 WP_134316771.1 4117820..4118497(+) (pilD) [Escherichia coli strain FDAARGOS_1283]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGGTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.721

100

0.387