Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   I6K13_RS02730 Genome accession   NZ_CP070162
Coordinates   582457..582897 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain FDAARGOS_1283     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 577457..587897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K13_RS02705 (I6K13_02705) coaE 577947..578567 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  I6K13_RS02710 (I6K13_02710) - 578592..578636 (+) 45 WP_120795372.1 protein YacM -
  I6K13_RS02715 (I6K13_02715) guaC 578792..579835 (+) 1044 WP_001217338.1 GMP reductase -
  I6K13_RS02720 (I6K13_02720) hofC 579870..581072 (-) 1203 WP_021523587.1 protein transport protein HofC -
  I6K13_RS02725 (I6K13_02725) pilB 581062..582447 (-) 1386 WP_001025180.1 type II secretion system protein GspE Machinery gene
  I6K13_RS02730 (I6K13_02730) pilA 582457..582897 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  I6K13_RS02735 (I6K13_02735) nadC 583101..583994 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I6K13_RS02740 (I6K13_02740) ampD 584082..584633 (+) 552 WP_000923733.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I6K13_RS02745 (I6K13_02745) ampE 584630..585484 (+) 855 WP_000172010.1 beta-lactamase regulator AmpE -
  I6K13_RS02750 (I6K13_02750) aroP 585527..586897 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=473357 I6K13_RS02730 WP_000360914.1 582457..582897(-) (pilA) [Escherichia coli strain FDAARGOS_1283]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=473357 I6K13_RS02730 WP_000360914.1 582457..582897(-) (pilA) [Escherichia coli strain FDAARGOS_1283]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363