Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   I6K11_RS12860 Genome accession   NZ_CP070134
Coordinates   2436990..2438486 (-) Length   498 a.a.
NCBI ID   WP_001040615.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1281     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2431990..2443486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K11_RS12835 (I6K11_12845) panM 2432211..2432594 (+) 384 WP_000778790.1 aspartate 1-decarboxylase autocleavage activator PanM -
  I6K11_RS12840 (I6K11_12850) livJ 2432794..2433897 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  I6K11_RS12845 (I6K11_12855) rpoH 2434169..2435023 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  I6K11_RS12850 (I6K11_12860) ftsX 2435268..2436326 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  I6K11_RS12855 (I6K11_12865) ftsE 2436319..2436987 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  I6K11_RS12860 (I6K11_12870) pilA 2436990..2438486 (-) 1497 WP_001040615.1 signal recognition particle-docking protein FtsY Machinery gene
  I6K11_RS12865 (I6K11_12875) rsmD 2438636..2439232 (+) 597 WP_001521506.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  I6K11_RS12870 (I6K11_12880) yhhL 2439222..2439491 (+) 270 WP_001295207.1 DUF1145 family protein -
  I6K11_RS12875 (I6K11_12885) yhhM 2439494..2439853 (-) 360 WP_000042886.1 DUF2500 domain-containing protein -
  I6K11_RS12880 (I6K11_12890) yhhN 2439994..2440620 (+) 627 WP_000964718.1 lysoplasmalogenase -
  I6K11_RS12885 (I6K11_12895) zntA 2440694..2442892 (+) 2199 WP_001521507.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  I6K11_RS12890 (I6K11_12900) tusA 2442995..2443240 (-) 246 WP_000130615.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54649.46 Da        Isoelectric Point: 4.1639

>NTDB_id=473054 I6K11_RS12860 WP_001040615.1 2436990..2438486(-) (pilA) [Escherichia coli strain FDAARGOS_1281]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVPAQEPAKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=473054 I6K11_RS12860 WP_001040615.1 2436990..2438486(-) (pilA) [Escherichia coli strain FDAARGOS_1281]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCCGGCGCAAGAGCCTGCGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTTGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTAGCTAT
CGAGCGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGATGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTTGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACTGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378