Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H4O16_RS20855 Genome accession   NZ_CP059975
Coordinates   4037151..4037585 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain G03     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4032151..4042585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H4O16_RS20840 (H4O16_20665) - 4033329..4033886 (+) 558 WP_000062063.1 PadR family transcriptional regulator -
  H4O16_RS20845 (H4O16_20670) metE 4034398..4036686 (+) 2289 WP_001007605.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  H4O16_RS20850 (H4O16_20675) comJ 4036725..4037114 (-) 390 WP_000424030.1 competence protein ComJ -
  H4O16_RS20855 (H4O16_20680) nucA/comI 4037151..4037585 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  H4O16_RS20860 (H4O16_20685) - 4037812..4038561 (+) 750 WP_000388472.1 DUF3967 domain-containing protein -
  H4O16_RS20865 (H4O16_20690) kinB 4038600..4039874 (-) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  H4O16_RS20870 (H4O16_20695) - 4040185..4040385 (+) 201 WP_000929277.1 hypothetical protein -
  H4O16_RS20875 (H4O16_20700) - 4040508..4041671 (+) 1164 WP_000434578.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=473038 H4O16_RS20855 WP_000811494.1 4037151..4037585(-) (nucA/comI) [Bacillus thuringiensis strain G03]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=473038 H4O16_RS20855 WP_000811494.1 4037151..4037585(-) (nucA/comI) [Bacillus thuringiensis strain G03]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGTGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549