Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   I6K22_RS23155 Genome accession   NZ_CP070124
Coordinates   4821530..4821970 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain FDAARGOS_1292     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4816530..4826970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K22_RS23135 (I6K22_23135) aroP 4817530..4818900 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  I6K22_RS23140 (I6K22_23140) ampE 4818943..4819797 (-) 855 WP_000171983.1 beta-lactamase regulator AmpE -
  I6K22_RS23145 (I6K22_23145) ampD 4819794..4820345 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  I6K22_RS23150 (I6K22_23150) nadC 4820433..4821326 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  I6K22_RS23155 (I6K22_23155) pilA 4821530..4821970 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  I6K22_RS23160 (I6K22_23160) pilB 4821980..4823365 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  I6K22_RS23165 (I6K22_23165) hofC 4823355..4824557 (+) 1203 WP_000157254.1 protein transport protein HofC -
  I6K22_RS23170 (I6K22_23170) guaC 4824592..4825635 (-) 1044 WP_001217338.1 GMP reductase -
  I6K22_RS23175 (I6K22_23175) - 4825791..4825835 (-) 45 WP_120795372.1 protein YacM -
  I6K22_RS23180 (I6K22_23180) coaE 4825860..4826480 (+) 621 WP_001269535.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=473011 I6K22_RS23155 WP_000360914.1 4821530..4821970(+) (pilA) [Escherichia coli strain FDAARGOS_1292]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=473011 I6K22_RS23155 WP_000360914.1 4821530..4821970(+) (pilA) [Escherichia coli strain FDAARGOS_1292]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363