Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   R2846_RS01430 Genome accession   NC_017452
Coordinates   275397..276089 (+) Length   230 a.a.
NCBI ID   WP_014550482.1    Uniprot ID   -
Organism   Haemophilus influenzae R2846     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 270397..281089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2846_RS01410 (R2846_0279) ampD 271671..272231 (-) 561 WP_014550480.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R2846_RS01415 (R2846_0280) pilA 272346..272795 (+) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  R2846_RS01420 (R2846_0281) pilB 272792..274186 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  R2846_RS01425 (R2846_0282) pilC 274183..275400 (+) 1218 WP_014550481.1 type II secretion system F family protein Machinery gene
  R2846_RS01430 (R2846_0283) pilD 275397..276089 (+) 693 WP_014550482.1 prepilin peptidase Machinery gene
  R2846_RS01435 (R2846_0284) rho 276143..277405 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  R2846_RS01440 (R2846_0285) metJ 277653..277970 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  R2846_RS01445 (R2846_0286) cueR 277984..278370 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  R2846_RS01450 (R2846_0287) - 278447..278653 (+) 207 WP_005666692.1 heavy-metal-associated domain-containing protein -
  R2846_RS01455 (R2846_0288) - 278739..278945 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  R2846_RS01460 (R2846_0289) - 279019..279225 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  R2846_RS01465 (R2846_0290) - 279299..279505 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26683.67 Da        Isoelectric Point: 8.5626

>NTDB_id=47296 R2846_RS01430 WP_014550482.1 275397..276089(+) (pilD) [Haemophilus influenzae R2846]
MIYFTMFLLGGILGIALWFYLSGFITRLQQEIYATYIELFPQNFLSFQPSVSSIQQKKCGHILRYFLNVGVEFIFLQIAF
KDSTFAIWLGLTLLILWAISYLDWHYQLISTTLCLWLLTIGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAVIIYFVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=47296 R2846_RS01430 WP_014550482.1 275397..276089(+) (pilD) [Haemophilus influenzae R2846]
ATGATTTACTTCACAATGTTTTTATTAGGCGGTATCTTAGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACCCG
TTTACAGCAAGAGATTTATGCGACTTATATTGAATTATTTCCACAAAACTTTCTGTCATTTCAGCCTAGCGTTTCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAATGTTGGGGTAGAATTTATATTTTTACAAATTGCCTTC
AAGGACTCTACTTTCGCTATATGGCTAGGGCTCACACTTCTTATCCTTTGGGCAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACTACACTCTGTTTATGGCTACTTACAATCGGTTTATTTGGAGCAGACAATAATTTTTCATTGCTTACGT
TATCTGAAAGCATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATTCTATTACGGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTTCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

89.565

100

0.896
  pilD Haemophilus influenzae Rd KW20

89.13

100

0.891

Multiple sequence alignment