Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6K28_RS09670 Genome accession   NZ_CP070115
Coordinates   2029082..2029759 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain FDAARGOS_1298     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2024082..2034759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K28_RS09655 (I6K28_09635) chiA 2025440..2028142 (+) 2703 WP_000773166.1 bifunctional chitinase/lysozyme -
  I6K28_RS09660 (I6K28_09640) bfd 2028311..2028505 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6K28_RS09665 (I6K28_09645) bfr 2028577..2029053 (+) 477 WP_000675504.1 bacterioferritin -
  I6K28_RS09670 (I6K28_09650) pilD 2029082..2029759 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  I6K28_RS09675 (I6K28_09655) gspM 2029759..2030220 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  I6K28_RS09680 (I6K28_09660) gspL 2030217..2031380 (-) 1164 WP_001332436.1 type II secretion system protein GspL -
  I6K28_RS09685 (I6K28_09665) gspK 2031395..2032378 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  I6K28_RS09690 (I6K28_09670) gspJ 2032371..2032958 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  I6K28_RS09695 (I6K28_09675) gspI 2032951..2033346 (-) 396 WP_001041747.1 type II secretion system minor pseudopilin GspI -
  I6K28_RS09700 (I6K28_09680) gspH 2033343..2033852 (-) 510 WP_001076038.1 type II secretion system minor pseudopilin GspH -
  I6K28_RS09705 (I6K28_09685) gspG 2033860..2034297 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=472884 I6K28_RS09670 WP_000178175.1 2029082..2029759(-) (pilD) [Escherichia coli strain FDAARGOS_1298]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=472884 I6K28_RS09670 WP_000178175.1 2029082..2029759(-) (pilD) [Escherichia coli strain FDAARGOS_1298]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387