Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6K07_RS04995 Genome accession   NZ_CP070103
Coordinates   1022001..1022678 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1277     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1017001..1027678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K07_RS04960 (I6K07_04960) gspG 1017481..1017918 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  I6K07_RS04965 (I6K07_04965) gspH 1017926..1018435 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  I6K07_RS04970 (I6K07_04970) gspI 1018432..1018809 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  I6K07_RS04975 (I6K07_04975) gspJ 1018802..1019389 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  I6K07_RS04980 (I6K07_04980) gspK 1019382..1020365 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  I6K07_RS04985 (I6K07_04985) gspL 1020380..1021543 (+) 1164 WP_001115235.1 type II secretion system protein GspL -
  I6K07_RS04990 (I6K07_04990) gspM 1021540..1022001 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  I6K07_RS04995 (I6K07_04995) pilD 1022001..1022678 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  I6K07_RS05000 (I6K07_05000) bfr 1022707..1023183 (-) 477 WP_000675504.1 bacterioferritin -
  I6K07_RS05005 (I6K07_05005) bfd 1023255..1023449 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6K07_RS05010 (I6K07_05010) chiA 1023618..1026311 (-) 2694 WP_000773141.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=472693 I6K07_RS04995 WP_000178161.1 1022001..1022678(+) (pilD) [Escherichia coli strain FDAARGOS_1277]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=472693 I6K07_RS04995 WP_000178161.1 1022001..1022678(+) (pilD) [Escherichia coli strain FDAARGOS_1277]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378