Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   H5A30_RS00395 Genome accession   NZ_CP059957
Coordinates   83513..84895 (+) Length   460 a.a.
NCBI ID   WP_014914021.1    Uniprot ID   A0A3S0ZXC5
Organism   Pectobacterium brasiliense strain IPO:4071 NAK:240     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 78513..89895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5A30_RS00375 (H5A30_00375) prfC 78816..80405 (-) 1590 WP_014914025.1 peptide chain release factor 3 -
  H5A30_RS00380 (H5A30_00380) - 80697..81614 (+) 918 WP_119871122.1 hypothetical protein -
  H5A30_RS00385 (H5A30_00385) - 81663..82361 (-) 699 WP_039510184.1 YtjB family periplasmic protein -
  H5A30_RS00390 (H5A30_00390) serB 82518..83495 (+) 978 WP_039282511.1 phosphoserine phosphatase -
  H5A30_RS00395 (H5A30_00395) radA 83513..84895 (+) 1383 WP_014914021.1 DNA repair protein RadA Machinery gene
  H5A30_RS00400 (H5A30_00400) nadR 85014..86267 (+) 1254 WP_039282517.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  H5A30_RS00405 (H5A30_00405) - 86264..86911 (-) 648 WP_119871121.1 5-formyltetrahydrofolate cyclo-ligase -
  H5A30_RS00415 (H5A30_00415) zapA 87209..87538 (-) 330 WP_010284667.1 cell division protein ZapA -
  H5A30_RS00420 (H5A30_00420) - 87731..88318 (+) 588 WP_039548009.1 YecA family protein -
  H5A30_RS00425 (H5A30_00425) pepP 88376..89701 (+) 1326 WP_094370312.1 Xaa-Pro aminopeptidase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49620.19 Da        Isoelectric Point: 7.4218

>NTDB_id=472582 H5A30_RS00395 WP_014914021.1 83513..84895(+) (radA) [Pectobacterium brasiliense strain IPO:4071 NAK:240]
MAKAVKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLASASTSRSDRLTGYAGESAGVSRVQKLSEISLEALPRFSTG
FQEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLSENMKTLYVTGEESLQQVAMRAHRLNLPTQNLNMLSETSIE
QICLIAEQEQPKLMVIDSIQVMHLADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREISNPSAIFLSRGDEITSGSSVMVVWEGTRPLLVEIQALVDQ
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLSLVSSFRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERITEAAKHGFKRAIVPHANMPKKVPASMQVFGVKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=472582 H5A30_RS00395 WP_014914021.1 83513..84895(+) (radA) [Pectobacterium brasiliense strain IPO:4071 NAK:240]
GTGGCAAAAGCCGTCAAACGGGCGTTTGTATGTAATGAATGCGGGGCTGACTACCCGCGCTGGCAAGGGCAGTGCAGCGC
CTGCCATGCCTGGAACACCATTACCGAAGTCCGTCTGGCCTCGGCGTCAACATCACGTTCCGACCGTCTCACCGGCTATG
CGGGCGAAAGCGCTGGCGTCAGCCGGGTACAAAAGCTCTCGGAAATCAGTCTTGAAGCCCTGCCGCGCTTTTCCACCGGT
TTTCAGGAGTTTGACCGCGTTCTGGGCGGCGGCGTCGTTCCGGGCAGCGCGATTCTGATCGGCGGTAACCCTGGCGCGGG
TAAAAGTACCTTACTGCTGCAAACGCTCTGCAAGCTGTCAGAGAACATGAAAACCCTGTACGTCACTGGGGAAGAATCCT
TGCAGCAGGTGGCGATGCGGGCGCATCGTCTCAACCTGCCGACCCAAAATCTCAATATGCTGTCGGAAACCAGCATCGAA
CAGATTTGCCTGATTGCCGAGCAGGAACAGCCGAAGCTGATGGTGATCGACTCCATTCAGGTGATGCATCTCGCGGATAT
TCAATCGTCACCCGGCAGCGTGGCGCAGGTGCGTGAAACCGCCGCCTACCTGACGCGCTTTGCAAAAACGCGCGGCGTCG
CCATCGTGATGGTCGGCCACGTCACCAAAGACGGTTCACTCGCCGGACCAAAAGTATTGGAACACTGCATCGACTGCTCC
GTGCTGCTGGATGGCGATGCCGATTCGCGCTTCCGCACCCTGCGCAGCCATAAAAACCGCTTCGGTGCCGTTAACGAGCT
GGGCGTGTTCGCGATGACGGAACAGGGTCTACGCGAGATCAGCAATCCGTCGGCAATTTTCCTCAGTCGCGGTGACGAAA
TCACGTCCGGCAGCTCGGTCATGGTGGTATGGGAAGGTACCCGTCCGCTGCTGGTCGAAATTCAGGCGCTGGTGGATCAA
TCGATGATGGCTAACCCGCGTCGCGTGGCGGTCGGGCTGGAGCAAAATCGCCTGGCGATCCTGCTGGCGGTATTACATCG
CCATGGCGGCTTGCAGATGTCCGATCAGGATGTGTTCGTGAATGTCGTCGGCGGCGTTAAAGTCACCGAAACCAGCGCCG
ACCTGGCGCTGCTGCTATCGCTGGTCTCCAGCTTCCGCGACCGCCCGTTGCCGCAAGATCTTGTCATCTTCGGTGAAGTC
GGTCTGGCGGGTGAAATCCGCCCTGTTCCGAGCGGCCAAGAGCGGATTACCGAAGCCGCCAAGCACGGCTTCAAACGCGC
CATCGTTCCTCATGCCAATATGCCGAAGAAAGTCCCTGCCAGCATGCAGGTGTTCGGCGTGAAAAAGCTGGCCGATGCGC
TGGCGATCCTCGACGATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(89-225)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S0ZXC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.261

100

0.483
  radA Streptococcus pneumoniae Rx1

42.982

99.13

0.426
  radA Streptococcus pneumoniae D39

42.982

99.13

0.426
  radA Streptococcus pneumoniae R6

42.982

99.13

0.426
  radA Streptococcus pneumoniae TIGR4

42.982

99.13

0.426
  radA Streptococcus mitis NCTC 12261

42.982

99.13

0.426
  radA Streptococcus mitis SK321

42.982

99.13

0.426