Detailed information NTG0472290

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: H4K32_RS09985
• Genome accession: NZ_CP059855
• Coordinates: 1922475..1923620 (-)
• Length: 381 a.a.
• NCBI ID: WP_003151067.1
• Uniprot ID: A0A6A8LBV3
• Organism: Bacillus velezensis strain Pm9
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 1917475..1928620

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H4K32_RS09945	speE	1917766..1918596 (+)	831	WP_003151052.1	spermidine synthase	-
H4K32_RS09950	speB	1918656..1919528 (+)	873	WP_003151054.1	agmatinase	-
H4K32_RS09955	-	1919698..1919916 (+)	219	WP_003151056.1	LuxR C-terminal-related transcriptional regulator	-
H4K32_RS09960	-	1920507..1921019 (-)	513	WP_032859656.1	stage II sporulation protein M	-
H4K32_RS09965	-	1921063..1921344 (-)	282	WP_003151060.1	hypothetical protein	-
H4K32_RS09970	-	1921753..1921956 (-)	204	WP_003151062.1	hypothetical protein	-
H4K32_RS09975	-	1921970..1922173 (-)	204	WP_003151064.1	helix-turn-helix transcriptional regulator	-
H4K32_RS09980	-	1922372..1922491 (-)	120	WP_003151066.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
H4K32_RS09985	rapF	1922475..1923620 (-)	1146	WP_003151067.1	tetratricopeptide repeat protein	Regulator
H4K32_RS09990	-	1923912..1924340 (+)	429	WP_003151069.1	DUF1934 domain-containing protein	-
H4K32_RS09995	argS	1924337..1926007 (+)	1671	WP_003151071.1	arginine--tRNA ligase	-
H4K32_RS10000	narK	1926142..1927332 (+)	1191	WP_044052814.1	nitrate transporter NarK	-
H4K32_RS10005	-	1927430..1928146 (+)	717	WP_014306017.1	Crp/Fnr family transcriptional regulator	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45339.49 Da
• Isoelectric Point: 4.9293

>NTDB_id=472290 H4K32_RS09985 WP_003151067.1 1922475..1923620(-) (rapF) [Bacillus velezensis strain Pm9]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide

• Length: 1146 bp

>NTDB_id=472290 H4K32_RS09985 WP_003151067.1 1922475..1923620(-) (rapF) [Bacillus velezensis strain Pm9]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACACGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAACGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

• Tetratricopeptide repeat (TPR_12) (223-287)

3D structure

• AlphaFold DB: A0A6A8LBV3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	74.803	100	0.748
rapC	Bacillus subtilis subsp. subtilis str. 168	53.158	99.738	0.53