Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H4K32_RS06580 Genome accession   NZ_CP059855
Coordinates   1271365..1271802 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain Pm9     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1266365..1276802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H4K32_RS06565 - 1269223..1269585 (-) 363 WP_014304320.1 helix-turn-helix domain-containing protein -
  H4K32_RS06570 hxlA 1269817..1270452 (+) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  H4K32_RS06575 hxlB 1270449..1271006 (+) 558 WP_077721772.1 6-phospho-3-hexuloisomerase -
  H4K32_RS06580 nucA/comI 1271365..1271802 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  H4K32_RS06585 nin/comJ 1271824..1272222 (+) 399 WP_014304317.1 competence protein ComJ Regulator
  H4K32_RS06590 - 1272263..1272481 (-) 219 Protein_1294 family 1 glycosylhydrolase -
  H4K32_RS20385 - 1272480..1272758 (-) 279 WP_107890409.1 family 1 glycosylhydrolase -
  H4K32_RS06600 - 1272700..1272975 (-) 276 WP_375140154.1 YckD family protein -
  H4K32_RS06605 - 1273093..1273527 (-) 435 WP_003156592.1 RDD family protein -
  H4K32_RS06610 - 1273542..1274678 (-) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  H4K32_RS06615 - 1274743..1275945 (-) 1203 WP_182275148.1 GTP-binding protein -
  H4K32_RS19685 - 1276098..1276223 (+) 126 Protein_1300 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=472279 H4K32_RS06580 WP_003156588.1 1271365..1271802(+) (nucA/comI) [Bacillus velezensis strain Pm9]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=472279 H4K32_RS06580 WP_003156588.1 1271365..1271802(+) (nucA/comI) [Bacillus velezensis strain Pm9]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641