Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6K67_RS03260 Genome accession   NZ_CP070026
Coordinates   589197..589874 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1337     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 584197..594874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K67_RS03225 (I6K67_03225) gspG 584677..585114 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  I6K67_RS03230 (I6K67_03230) gspH 585122..585631 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  I6K67_RS03235 (I6K67_03235) gspI 585628..586005 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  I6K67_RS03240 (I6K67_03240) gspJ 585998..586585 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  I6K67_RS03245 (I6K67_03245) gspK 586578..587561 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  I6K67_RS03250 (I6K67_03250) gspL 587576..588739 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  I6K67_RS03255 (I6K67_03255) gspM 588736..589197 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  I6K67_RS03260 (I6K67_03260) pilD 589197..589874 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  I6K67_RS03265 (I6K67_03265) bfr 589903..590379 (-) 477 WP_000675504.1 bacterioferritin -
  I6K67_RS03270 (I6K67_03270) bfd 590451..590645 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6K67_RS03275 (I6K67_03275) chiA 590814..593532 (-) 2719 Protein_575 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=471925 I6K67_RS03260 WP_000178161.1 589197..589874(+) (pilD) [Escherichia coli strain FDAARGOS_1337]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=471925 I6K67_RS03260 WP_000178161.1 589197..589874(+) (pilD) [Escherichia coli strain FDAARGOS_1337]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378