Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6L03_RS20725 Genome accession   NZ_CP069945
Coordinates   4347575..4348252 (+) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain FDAARGOS_1373     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4342575..4353252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L03_RS20690 (I6L03_20690) gspG 4343055..4343492 (+) 438 WP_001765226.1 type II secretion system major pseudopilin GspG -
  I6L03_RS20695 (I6L03_20695) gspH 4343500..4344009 (+) 510 WP_001076035.1 type II secretion system minor pseudopilin GspH -
  I6L03_RS20700 (I6L03_20700) gspI 4344006..4344383 (+) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  I6L03_RS20705 (I6L03_20705) gspJ 4344376..4344963 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  I6L03_RS20710 (I6L03_20710) gspK 4344956..4345939 (+) 984 WP_001765225.1 type II secretion system minor pseudopilin GspK -
  I6L03_RS20715 (I6L03_20715) gspL 4345954..4347117 (+) 1164 WP_001765224.1 type II secretion system protein GspL -
  I6L03_RS20720 (I6L03_20720) gspM 4347114..4347575 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  I6L03_RS20725 (I6L03_20725) pilD 4347575..4348252 (+) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  I6L03_RS20730 (I6L03_20730) bfr 4348281..4348757 (-) 477 WP_000675504.1 bacterioferritin -
  I6L03_RS20735 (I6L03_20735) bfd 4348829..4349023 (-) 195 WP_001765223.1 bacterioferritin-associated ferredoxin -
  I6L03_RS20740 (I6L03_20740) chiA 4349192..4351894 (-) 2703 WP_001765222.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=471301 I6L03_RS20725 WP_000178175.1 4347575..4348252(+) (pilD) [Escherichia coli strain FDAARGOS_1373]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=471301 I6L03_RS20725 WP_000178175.1 4347575..4348252(+) (pilD) [Escherichia coli strain FDAARGOS_1373]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387