Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6K32_RS15985 Genome accession   NZ_CP069935
Coordinates   3260266..3260943 (+) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain FDAARGOS_1302     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3255266..3265943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K32_RS15950 (I6K32_15950) gspG 3255728..3256165 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  I6K32_RS15955 (I6K32_15955) gspH 3256173..3256682 (+) 510 WP_001076038.1 type II secretion system minor pseudopilin GspH -
  I6K32_RS15960 (I6K32_15960) gspI 3256679..3257074 (+) 396 WP_001041747.1 type II secretion system minor pseudopilin GspI -
  I6K32_RS15965 (I6K32_15965) gspJ 3257067..3257654 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  I6K32_RS15970 (I6K32_15970) gspK 3257647..3258630 (+) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  I6K32_RS15975 (I6K32_15975) gspL 3258645..3259808 (+) 1164 WP_001332436.1 type II secretion system protein GspL -
  I6K32_RS15980 (I6K32_15980) gspM 3259805..3260266 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  I6K32_RS15985 (I6K32_15985) pilD 3260266..3260943 (+) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  I6K32_RS15990 (I6K32_15990) bfr 3260972..3261448 (-) 477 WP_000675504.1 bacterioferritin -
  I6K32_RS15995 (I6K32_15995) bfd 3261520..3261714 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6K32_RS16000 (I6K32_16000) chiA 3261883..3264585 (-) 2703 WP_000773166.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=471190 I6K32_RS15985 WP_000178175.1 3260266..3260943(+) (pilD) [Escherichia coli strain FDAARGOS_1302]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=471190 I6K32_RS15985 WP_000178175.1 3260266..3260943(+) (pilD) [Escherichia coli strain FDAARGOS_1302]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387