Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6K16_RS09570 Genome accession   NZ_CP069893
Coordinates   1964416..1965093 (+) Length   225 a.a.
NCBI ID   WP_000178169.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1286     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1959416..1970093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K16_RS09535 (I6K16_09535) gspG 1959896..1960333 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  I6K16_RS09540 (I6K16_09540) gspH 1960341..1960850 (+) 510 WP_001076031.1 type II secretion system minor pseudopilin GspH -
  I6K16_RS09545 (I6K16_09545) gspI 1960847..1961224 (+) 378 WP_001041738.1 type II secretion system minor pseudopilin GspI -
  I6K16_RS09550 (I6K16_09550) gspJ 1961217..1961804 (+) 588 WP_000610629.1 type II secretion system minor pseudopilin GspJ -
  I6K16_RS09555 (I6K16_09555) gspK 1961797..1962780 (+) 984 WP_001058528.1 type II secretion system minor pseudopilin GspK -
  I6K16_RS09560 (I6K16_09560) gspL 1962795..1963958 (+) 1164 WP_001115245.1 type II secretion system protein GspL -
  I6K16_RS09565 (I6K16_09565) gspM 1963955..1964416 (+) 462 WP_000597914.1 type II secretion system protein GspM -
  I6K16_RS09570 (I6K16_09570) pilD 1964416..1965093 (+) 678 WP_000178169.1 prepilin peptidase GspO Machinery gene
  I6K16_RS09575 (I6K16_09575) bfr 1965122..1965598 (-) 477 WP_000675505.1 bacterioferritin -
  I6K16_RS09580 (I6K16_09580) bfd 1965670..1965864 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6K16_RS09585 (I6K16_09585) chiA 1966033..1968735 (-) 2703 WP_000773176.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24812.67 Da        Isoelectric Point: 7.9585

>NTDB_id=471092 I6K16_RS09570 WP_000178169.1 1964416..1965093(+) (pilD) [Escherichia coli strain FDAARGOS_1286]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=471092 I6K16_RS09570 WP_000178169.1 1964416..1965093(+) (pilD) [Escherichia coli strain FDAARGOS_1286]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTAATTGCCTCACTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCGTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382