Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   H3L98_RS01300 Genome accession   NZ_CP059563
Coordinates   182563..183945 (+) Length   460 a.a.
NCBI ID   WP_027021985.1    Uniprot ID   A0AAE9HUU4
Organism   Conchiformibius steedae strain DSM 2580     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 177563..188945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L98_RS01270 (H3L98_01270) - 179324..179668 (-) 345 WP_027021989.1 helix-turn-helix domain-containing protein -
  H3L98_RS11025 - 179945..180088 (+) 144 Protein_181 excalibur calcium-binding domain-containing protein -
  H3L98_RS01290 (H3L98_01290) hemA 180452..181723 (-) 1272 WP_027021987.1 glutamyl-tRNA reductase -
  H3L98_RS01295 (H3L98_01295) - 181959..182327 (-) 369 WP_051532071.1 DUF7606 domain-containing protein -
  H3L98_RS01300 (H3L98_01300) radA 182563..183945 (+) 1383 WP_027021985.1 DNA repair protein RadA Machinery gene
  H3L98_RS01305 (H3L98_01305) - 184116..184646 (+) 531 WP_051532070.1 Imm6 family immunity protein -
  H3L98_RS01310 (H3L98_01310) - 184851..185594 (+) 744 WP_027021983.1 hypothetical protein -
  H3L98_RS01315 (H3L98_01315) rsfS 185753..186145 (-) 393 WP_027021982.1 ribosome silencing factor -
  H3L98_RS01320 (H3L98_01320) fmt 186230..187159 (-) 930 WP_027021981.1 methionyl-tRNA formyltransferase -
  H3L98_RS01325 (H3L98_01325) - 187374..188309 (+) 936 WP_156932286.1 hypothetical protein -
  H3L98_RS01330 (H3L98_01330) - 188379..188795 (+) 417 WP_027021979.1 DUF2147 domain-containing protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49234.74 Da        Isoelectric Point: 7.6490

>NTDB_id=470640 H3L98_RS01300 WP_027021985.1 182563..183945(+) (radA) [Conchiformibius steedae strain DSM 2580]
MAKAPKTVYQCSECGGTAPKWQGKCPHCGEWNTLQESLAAPEPKNPRFQSWAAETAQVQPLAQVSAAEIPRQATGMGELD
RVLGGGLVDGAVILLGGDPGIGKSTLLLQTLALAAAQRKVLYVSGEESAQQVALRAQRLGVRAQGVDLLAEIRLEAVLAA
LKQHRPEIVVIDSIQTVYSDLITSAPGSVSQVRECAAQLTRSAKQMGIAIILVGHVTKDGAIAGPRVLEHMVDTVLYFEG
DPHSNHRMIRAIKNRFGAANELGVFAMTEQGLKGVSNPSAIFLASYRDDVSGSCVLVTQEGTRPLLVEIQALVDDAHGFT
PKRLTVGLEQNRLAMLLAVLNRHAGIACFDRDVFLNAVGGVKIGEPAADLAVILAMLSSYRNRPLPQKMVAFGEVGLGGE
IRPVPRGQERLKEAEKLGFRRAIVPKANLPRQTNEFPNLKIHGVESLAEAVALCRDGDNE

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=470640 H3L98_RS01300 WP_027021985.1 182563..183945(+) (radA) [Conchiformibius steedae strain DSM 2580]
ATGGCAAAAGCCCCCAAAACCGTTTACCAATGCAGCGAATGCGGCGGAACTGCACCCAAATGGCAAGGCAAATGCCCACA
TTGCGGCGAATGGAACACCTTGCAGGAAAGCCTTGCCGCGCCCGAACCGAAAAACCCGCGTTTTCAATCGTGGGCAGCAG
AAACCGCTCAAGTTCAACCTTTAGCACAAGTAAGCGCCGCCGAAATCCCGCGCCAAGCTACTGGTATGGGCGAACTGGAC
CGCGTACTCGGTGGCGGGCTGGTAGATGGCGCTGTGATTTTATTGGGTGGCGACCCTGGCATCGGCAAATCCACCCTACT
GCTGCAAACCCTCGCCTTGGCAGCCGCGCAGCGCAAAGTGCTGTATGTATCGGGCGAGGAATCCGCGCAACAAGTGGCAT
TGCGGGCGCAGCGTTTGGGCGTACGGGCGCAGGGTGTGGATTTGCTGGCAGAAATCCGTTTGGAAGCCGTGTTAGCCGCT
TTAAAGCAACATCGTCCCGAAATTGTTGTGATTGATTCCATTCAAACCGTGTATTCCGATTTGATTACTTCCGCCCCTGG
TTCGGTGTCACAAGTACGCGAGTGCGCCGCCCAGCTTACCCGCAGTGCCAAACAAATGGGTATTGCCATCATTTTAGTTG
GACATGTGACCAAAGACGGAGCGATTGCTGGTCCGCGCGTATTGGAACACATGGTGGATACCGTTTTATATTTTGAAGGC
GACCCACATTCCAACCACCGCATGATACGCGCCATCAAAAACCGTTTCGGTGCAGCCAACGAATTAGGCGTATTTGCCAT
GACCGAACAAGGCTTAAAAGGCGTATCCAACCCCTCTGCCATCTTTTTGGCGAGTTACCGCGACGACGTTTCCGGTTCTT
GCGTTTTGGTTACCCAAGAAGGCACACGCCCGCTGCTGGTAGAAATTCAAGCTTTGGTGGACGATGCCCACGGCTTTACC
CCCAAACGCCTGACAGTCGGCTTGGAACAAAACCGTTTAGCGATGCTGCTCGCCGTCTTAAACCGCCACGCAGGCATTGC
CTGTTTTGACCGCGATGTCTTTTTAAACGCCGTAGGCGGTGTGAAAATCGGCGAACCTGCTGCCGACTTGGCTGTGATTT
TAGCCATGCTGTCCAGCTATCGCAACCGCCCACTGCCGCAAAAAATGGTGGCGTTTGGTGAAGTGGGTTTGGGTGGCGAA
ATCCGTCCCGTCCCACGCGGGCAGGAACGCCTGAAAGAAGCCGAAAAACTCGGATTCCGCCGCGCCATCGTCCCCAAAGC
CAACCTACCCCGCCAAACCAACGAATTTCCCAACTTAAAAATTCACGGTGTAGAAAGCTTGGCAGAAGCCGTAGCATTGT
GCCGCGATGGAGATAATGAATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(366-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(78-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.559

98.696

0.489
  radA Streptococcus mitis SK321

48.124

98.478

0.474
  radA Streptococcus pneumoniae D39

48.009

98.261

0.472
  radA Streptococcus pneumoniae TIGR4

48.009

98.261

0.472
  radA Streptococcus pneumoniae R6

48.009

98.261

0.472
  radA Streptococcus pneumoniae Rx1

48.009

98.261

0.472
  radA Streptococcus mitis NCTC 12261

49.184

93.261

0.459