Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H2788_RS15970 Genome accession   NZ_CP059550
Coordinates   3367975..3368775 (-) Length   266 a.a.
NCBI ID   WP_004698762.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain E-022166     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3362975..3373775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2788_RS15950 (H2788_15860) - 3364040..3364738 (-) 699 WP_002053293.1 tetratricopeptide repeat protein -
  H2788_RS15955 (H2788_15865) hisS 3364767..3366059 (-) 1293 WP_347016585.1 histidine--tRNA ligase -
  H2788_RS15960 (H2788_15870) ispG 3366056..3367171 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  H2788_RS15965 (H2788_15875) - 3367193..3367984 (-) 792 WP_347016586.1 RodZ domain-containing protein -
  H2788_RS15970 (H2788_15880) pilF 3367975..3368775 (-) 801 WP_004698762.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H2788_RS15975 (H2788_15885) rlmN 3368791..3370023 (-) 1233 WP_004698763.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  H2788_RS15980 (H2788_15890) ndk 3370146..3370577 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  H2788_RS15985 (H2788_15895) iscX 3370691..3370891 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  H2788_RS15990 (H2788_15900) - 3370991..3371542 (-) 552 WP_019458079.1 phosphatase PAP2 family protein -
  H2788_RS15995 (H2788_15905) - 3371567..3372865 (-) 1299 WP_347017515.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30125.50 Da        Isoelectric Point: 9.2307

>NTDB_id=470545 H2788_RS15970 WP_004698762.1 3367975..3368775(-) (pilF) [Acinetobacter seifertii strain E-022166]
MSTPKLKIMLCMGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=470545 H2788_RS15970 WP_004698762.1 3367975..3368775(-) (pilF) [Acinetobacter seifertii strain E-022166]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCAGTTGCATTTCTGGCAAGTGGTTGTCAGACGTCGCC
AACACTAAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCTGCAGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAACGTTCCCTCGACCAAGCTCTAAGTATCGAGAGTCGTGACGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACACTATTTTAAACGTGCAATTTCTTCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTATACCAAATGGAACGTTATAATGATGCTGTCGAGCAGTTCCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGTTACCAAGCCTTAGAAAATCTGGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCGCTCCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCAGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAATCAA
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.744

100

0.977
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726