Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H2789_RS15795 Genome accession   NZ_CP059549
Coordinates   3343493..3344293 (-) Length   266 a.a.
NCBI ID   WP_347018945.1    Uniprot ID   -
Organism   Acinetobacter calcoaceticus strain NCIMB13260     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3338493..3349293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2789_RS15775 (H2789_15755) - 3339558..3340256 (-) 699 WP_347018944.1 tetratricopeptide repeat protein -
  H2789_RS15780 (H2789_15760) hisS 3340285..3341577 (-) 1293 WP_017388395.1 histidine--tRNA ligase -
  H2789_RS15785 (H2789_15765) ispG 3341574..3342689 (-) 1116 WP_004641112.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  H2789_RS15790 (H2789_15770) - 3342711..3343502 (-) 792 WP_004641111.1 helix-turn-helix domain-containing protein -
  H2789_RS15795 (H2789_15775) pilF 3343493..3344293 (-) 801 WP_347018945.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H2789_RS15800 (H2789_15780) rlmN 3344308..3345543 (-) 1236 WP_005036936.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  H2789_RS15805 (H2789_15785) ndk 3345667..3346098 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  H2789_RS15810 (H2789_15790) iscX 3346212..3346412 (-) 201 WP_003654869.1 Fe-S cluster assembly protein IscX -
  H2789_RS15815 (H2789_15795) - 3346512..3347063 (-) 552 WP_005044865.1 phosphatase PAP2 family protein -
  H2789_RS15820 (H2789_15800) - 3347088..3348377 (-) 1290 WP_347020250.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30120.30 Da        Isoelectric Point: 9.0417

>NTDB_id=470504 H2789_RS15795 WP_347018945.1 3343493..3344293(-) (pilF) [Acinetobacter calcoaceticus strain NCIMB13260]
MSTPKLKTVFCMGVAVALLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDVIEQFRIAGTTLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQSLLANRDSYISMLELAEIFYLQQQIPAATQMYEQYARTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=470504 H2789_RS15795 WP_347018945.1 3343493..3344293(-) (pilF) [Acinetobacter calcoaceticus strain NCIMB13260]
TTGAGTACTCCAAAGTTAAAAACGGTATTTTGTATGGGAGTTGCAGTTGCGCTTTTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAAAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCAGGTGACTTGG
ATTCGGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
CTACAGCAAGAAGGTAGTAAGCCGAACTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAGCCAGATAA
TGCTCAAGCACATAATAACTATGGTACTTATTTATACCAAATGGAACGCTATAATGATGTGATCGAGCAATTTCGTATTG
CTGGCACAACATTAGGTTATGACCAACGCTATCAGGCGTTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAGCAATCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGCTCGCACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAA
TTACGTGCCTTGTTCCCAGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-124)

Tetratricopeptide repeat (TPR_16)

(149-205)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

74.615

97.744

0.729