Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   H2784_RS15215 Genome accession   NZ_CP059547
Coordinates   3266027..3266827 (-) Length   266 a.a.
NCBI ID   WP_193759251.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain 10_3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3261027..3271827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2784_RS15195 (H2784_15195) - 3262092..3262790 (-) 699 WP_000058788.1 YfgM family protein -
  H2784_RS15200 (H2784_15200) hisS 3262819..3264111 (-) 1293 WP_000095252.1 histidine--tRNA ligase -
  H2784_RS15205 (H2784_15205) ispG 3264108..3265223 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  H2784_RS15210 (H2784_15210) - 3265245..3266036 (-) 792 WP_000405161.1 helix-turn-helix domain-containing protein -
  H2784_RS15215 (H2784_15215) pilF 3266027..3266827 (-) 801 WP_193759251.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H2784_RS15220 (H2784_15220) rlmN 3266843..3268075 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  H2784_RS15225 (H2784_15225) ndk 3268198..3268629 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  H2784_RS15230 (H2784_15230) iscX 3268743..3268943 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  H2784_RS15235 (H2784_15235) - 3269043..3269594 (-) 552 WP_000669566.1 phosphatase PAP2 family protein -
  H2784_RS15240 (H2784_15240) - 3269619..3270917 (-) 1299 WP_001991191.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30110.44 Da        Isoelectric Point: 9.2307

>NTDB_id=470414 H2784_RS15215 WP_193759251.1 3266027..3266827(-) (pilF) [Acinetobacter baumannii strain 10_3]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=470414 H2784_RS15215 WP_193759251.1 3266027..3266827(-) (pilF) [Acinetobacter baumannii strain 10_3]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTGTTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.248

100

0.992
  pilF Acinetobacter baylyi ADP1

73.846

97.744

0.722